A method for the isolation of &#945;-ribazole from vitamin B<sub>12</sub>, and its enzymatic conversion to &#945;-ribazole 5'-phosphate.

Malalasekara, Lahiru; Escalante-Semerena, Jorge C

A method for the isolation of α-ribazole from vitamin B₁₂, and its enzymatic conversion to α-ribazole 5'-phosphate.

Malalasekara, Lahiru; Escalante-Semerena, Jorge C.

Afiliación

Malalasekara L; Department of Microbiology, University of Georgia, Athens, GA, United States.
Escalante-Semerena JC; Department of Microbiology, University of Georgia, Athens, GA, United States. Electronic address: jcescala@uga.edu.

Methods Enzymol ; 668: 125-136, 2022.

Article en En | MEDLINE | ID: mdl-35589191

Asunto(s)

Fosfatos; Vitamina B 12; Cobalto/química; Cobamidas/química; Cobamidas/metabolismo; Coenzimas; Ligandos; Ribonucleósidos; Vitamina B 12/metabolismo; Vitaminas

Palabras clave

Boronate affinity chromatography; Coenzyme B(12); Late steps of coenzyme B(12) biosynthesis; Scale-up synthesis of α-ribazole; Vitamin B(12) hydrolysis; α-Ribazole kinase; α-Ribazole-phosphate

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fosfatos / Vitamina B 12 Idioma: En Revista: Methods Enzymol Año: 2022 Tipo del documento: Article País de afiliación: Estados Unidos

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