Effect of osmotic stress on live cell plasma membranes, probed via Laurdan general polarization measurements.
Biophys J
; 121(12): 2411-2418, 2022 06 21.
Article
en En
| MEDLINE
| ID: mdl-35596525
ABSTRACT
Here we seek to gain insight into changes in the plasma membrane of live cells upon the application of osmotic stress using Laurdan, a fluorescent probe that reports on membrane organization, hydration, and dynamics. It is known that the application of osmotic stress to lipid vesicles causes a decrease in Laurdan's generalized polarization (GP), which has been interpreted as an indication of membrane stretching. In cells, we see the opposite effects, as GP increases when the osmolarity of the solution is decreased. This increase in GP is associated with the presence of caveolae, which are known to disassemble and flatten in response to osmotic stress.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Lauratos
/
2-Naftilamina
Idioma:
En
Revista:
Biophys J
Año:
2022
Tipo del documento:
Article