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Multiple dsRNases Involved in Exogenous dsRNA Degradation of Fall Armyworm Spodoptera frugiperda.
Yao, Yang; Lin, Dong-Jiang; Cai, Xiang-Yun; Wang, Ran; Hou, You-Ming; Hu, Chao-Hua; Gao, San-Ji; Wang, Jin-Da.
Afiliación
  • Yao Y; National Engineering Research Center of Sugarcane, Fujian Agricultural and Forestry University, Fuzhou, China.
  • Lin DJ; National Engineering Research Center of Sugarcane, Fujian Agricultural and Forestry University, Fuzhou, China.
  • Cai XY; State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Ministry of Education, College of Plant Protection, Fujian Agricultural and Forestry University, Fuzhou, China.
  • Wang R; Institute of Plant Protection, Beijing Academy of Agriculture and Forestry, Beijing, China.
  • Hou YM; State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Ministry of Education, College of Plant Protection, Fujian Agricultural and Forestry University, Fuzhou, China.
  • Hu CH; National Engineering Research Center of Sugarcane, Fujian Agricultural and Forestry University, Fuzhou, China.
  • Gao SJ; National Engineering Research Center of Sugarcane, Fujian Agricultural and Forestry University, Fuzhou, China.
  • Wang JD; National Engineering Research Center of Sugarcane, Fujian Agricultural and Forestry University, Fuzhou, China.
Front Physiol ; 13: 850022, 2022.
Article en En | MEDLINE | ID: mdl-35600298
RNAi is regarded as a promising technology for pest control. However, not all insects are sensitive to RNAi. Studies have confirmed that insect dsRNases are one of key factors affecting RNAi efficiency. In the current study, we identified four genes coding for dsRNases from the Spodoptera frugiperda genome. Spatial and temporal expression analysis showed that those dsRNases were highly expressed in the midgut and old larvae. Then a delivery method was applied for inducing efficient RNAi based on dsRNA encapsulated by liposome. Furthermore, we assessed degradation efficiency by incubation with dsRNA with gut juice or hemocoel to characterize potential roles of different SfdsRNases after suppression of SfdsRNase. The result showed that interferenced with any sfdsRNase reduced the degradation of exogenous dsRNA in midgut, interfered with sfdsRNase1 and sfdsRNase3 slowed down the degradation of exogenous dsRNA in hemolymph. Our data suggest the evolutionary expansion and multiple high activity dsRNase genes would take part in the RNAi obstinate in S. frugiperda, besides we also provide an efficient RNAi method for better use of RNAi in S. frugiperda.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Prognostic_studies Idioma: En Revista: Front Physiol Año: 2022 Tipo del documento: Article País de afiliación: China Pais de publicación: Suiza

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Prognostic_studies Idioma: En Revista: Front Physiol Año: 2022 Tipo del documento: Article País de afiliación: China Pais de publicación: Suiza