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Oxysterols profiles in zebrafish (Danio rerio) embryos exposed to bisphenol A.
Vremere, Anton; Merola, Carmine; Fanti, Federico; Sergi, Manuel; Perugini, Monia; Compagnone, Dario; Mikhail, Milena; Lorenzetti, Stefano; Amorena, Michele.
Afiliación
  • Vremere A; Faculty of Bioscience and Technology for Food, Agriculture and Environment, University of Teramo, Italy; Dpt. of Food Safety, Nutrition and Veterinary Public Health, Istituto Superiore di Sanità - ISS, Rome, Italy. Electronic address: avremere@unite.it.
  • Merola C; Faculty of Bioscience and Technology for Food, Agriculture and Environment, University of Teramo, Italy. Electronic address: cmerola@unite.it.
  • Fanti F; Faculty of Bioscience and Technology for Food, Agriculture and Environment, University of Teramo, Italy. Electronic address: ffanti@unite.it.
  • Sergi M; Faculty of Bioscience and Technology for Food, Agriculture and Environment, University of Teramo, Italy. Electronic address: msergi@unite.it.
  • Perugini M; Faculty of Bioscience and Technology for Food, Agriculture and Environment, University of Teramo, Italy. Electronic address: mperugini@unite.it.
  • Compagnone D; Faculty of Bioscience and Technology for Food, Agriculture and Environment, University of Teramo, Italy. Electronic address: dcompagnone@unite.it.
  • Mikhail M; Dpt. of Food Safety, Nutrition and Veterinary Public Health, Istituto Superiore di Sanità - ISS, Rome, Italy. Electronic address: milena.mikhail@iss.it.
  • Lorenzetti S; Dpt. of Food Safety, Nutrition and Veterinary Public Health, Istituto Superiore di Sanità - ISS, Rome, Italy. Electronic address: stefano.lorenzetti@iss.it.
  • Amorena M; Faculty of Bioscience and Technology for Food, Agriculture and Environment, University of Teramo, Italy. Electronic address: mamorena@unite.it.
Food Chem Toxicol ; 165: 113166, 2022 Jul.
Article en En | MEDLINE | ID: mdl-35609738
ABSTRACT

INTRODUCTION:

Oxysterols are cholesterol oxidation products and bioactive lipids involved in developmental signalling pathways, embryonic and postembryonic tissue patterning and homeostasis. The embryonic period is a very sensitive window of exposure to bisphenol A (BPA), hence the role of BPA on the levels of oxysterols in the very early stages of zebrafish embryogenesis is a relevant novel field of investigation.

OBJECTIVES:

To compare the role of BPA on oxysterols levels in zebrafish embryos at 8 and 24 h post fertilization (hpf) with cytochromes P450 (CYPs)-modulating chemicals (carbamazepine, ketoconazole, and hydrogen peroxide).

METHODS:

Upon a dose range finding, zebrafish embryos were exposed to environmentally relevant (0.04 µM) and toxicological (17.5 µM) BPA concentrations. Seven oxysterols were profiled by high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS).

RESULTS:

Similarly to the CYPs-modulating chemicals, BPA caused i) no significant changes at 8 hpf and ii) a dose-dependent increase of total oxysterols at 24 hpf, with 27-hydroxycholesterol as the most regulated oxysterol.

DISCUSSION:

In the first day post-fertilization of the zebrafish embryos, the role of BPA alike a CYPs-modulating chemical was confirmed by the similar oxysterol changes observed with the already known CYPs-modulating chemicals.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Contaminantes Químicos del Agua / Oxiesteroles Límite: Animals Idioma: En Revista: Food Chem Toxicol Año: 2022 Tipo del documento: Article
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Contaminantes Químicos del Agua / Oxiesteroles Límite: Animals Idioma: En Revista: Food Chem Toxicol Año: 2022 Tipo del documento: Article