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An updated catalog of lipocalins of the chagas disease vector Rhodnius prolixus (Hemiptera, Reduviidae).
Santos, Daniela V; Gontijo, Nelder F; Pessoa, Garsielle C D; Sant'Anna, Mauricio R V; Araujo, Ricardo N; Pereira, Marcos H; Koerich, Leonardo B.
Afiliación
  • Santos DV; Laboratorio de Fisiologia de Insetos Hematofagos, Departamento de Parasitologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Brazil.
  • Gontijo NF; Laboratorio de Fisiologia de Insetos Hematofagos, Departamento de Parasitologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Brazil.
  • Pessoa GCD; Laboratorio de Fisiologia de Insetos Hematofagos, Departamento de Parasitologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Brazil.
  • Sant'Anna MRV; Laboratorio de Fisiologia de Insetos Hematofagos, Departamento de Parasitologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Brazil.
  • Araujo RN; Laboratorio de Fisiologia de Insetos Hematofagos, Departamento de Parasitologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Brazil.
  • Pereira MH; Laboratorio de Fisiologia de Insetos Hematofagos, Departamento de Parasitologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Brazil.
  • Koerich LB; Laboratorio de Fisiologia de Insetos Hematofagos, Departamento de Parasitologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Brazil. Electronic address: lbkoerich@ufmg.br.
Insect Biochem Mol Biol ; 146: 103797, 2022 07.
Article en En | MEDLINE | ID: mdl-35640811
ABSTRACT
The haematophagy process by arthropods has been one of the main targets of studies in the parasite-host interaction, and the kissing-bug Rhodnius prolixus, vector of the protozoan Trypanosoma cruzi, has been one of the main models for such studies. Still in the 1980s, it was identified that among the salivary proteins for disrupting vertebrate host homeostasis, lipocalins were among the most relevant proteins for this process. Since then, 30 lipocalins have been identified in the salivary glands of R. prolixus, that promotes vasodilatation, prevents inflammation, act as anticoagulants and inhibits platelet aggregation. The present work aims to identify new lipocalins from R. prolixus, combining transcriptome and genome data. Identified new genes were mapped and had their structure annotated. To infer an evolutionary relationship between lipocalins, and to support the predicted functions for each lipocalin, all amino acid sequences were used to construct phylogenetic trees. We identified a total of 29 new lipocalins, 3 new bioaminogenic-biding proteins (which act to inhibit platelet aggregation and vasodilation), 9 new inhibitors of platelet aggregation, 7 new apolipoproteins and 10 lipocalins with no putative function. In addition, we observed that several of the lipocalins are also expressed in different R. prolxius tissues, including gut, central nervous system, antennae, and reproductive organs. In addition to newly identified lipocalins and a mapping the new and old lipocalins in the genome of R. prolixus, our study also carried out a review on functional status and nomenclature of some of the already identified lipocalins. Our study reinforces that we are far from understanding the role of lipocalins in the physiology of R. prolixus, and that studies of this family are still of great relevance.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Rhodnius / Enfermedad de Chagas Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Insect Biochem Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR / BIOQUIMICA Año: 2022 Tipo del documento: Article País de afiliación: Brasil

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Rhodnius / Enfermedad de Chagas Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Insect Biochem Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR / BIOQUIMICA Año: 2022 Tipo del documento: Article País de afiliación: Brasil