Astragaloside IV attenuated TGF-ß1- induced epithelial-mesenchymal transition of renal tubular epithelial cells via connexin 43 and Akt/mTOR signaling pathway.
Tissue Cell
; 77: 101831, 2022 Aug.
Article
en En
| MEDLINE
| ID: mdl-35643056
ABSTRACT
INTRODUCTION:
The objective of the study was to observe whether connexin 43 (Cx43) could regulate epithelial mesenchymal transformation (EMT) of renal tubular epithelial cells (RTECs) by influencing Akt/mTOR signaling pathway, and whether ASV could inhibit the development of renal interstitial fibrosis by regulating Cx43.METHODS:
Lentivirus infection was transfected into RTECs with the final concentration of 50 ×PFU/ cell to regulate the expression of Cx43. And RTECs were intervened by different doses of Astragaloside IV (ASV). After synchronous culture of RTECs in each groupï¼the expression levels of EMT-related indicators and Cx43 were detected by fluorescence microscope and Western-Blotting (WB), even the protein expressions and phosphorylation levels of AKT and mTOR in different groups were detected by WB.RESULTS:
When the expression of Cx43 in RTECs was regulated by lentivirus infection, the degree of EMT induced by TGFß1 and the phosphorylation level of Akt and mTOR were changed accordingly, indicating that Akt/mTOR pathway might be a downstream molecular mechanism by which Cx43 could regulate EMT. After intervention with different doses of ASV, the expression level of Cx43 increased with obvious concentration dependence, and the expression levels of p-Akt and p- mTOR were significantly altered, suggesting that ASV could effectively increase the protein expressions of TGFß1-induced Cx43 in RTECs and inhibit the phosphorylation levels of Akt and mTOR.CONCLUSION:
Cx43 were the main material basis of RTECs' injury, and ASV could inhibit TGF-ß1- induced RTECs' transdifferentiation. In-depth study of the mechanism might provide a broad application prospect for the treatment of renal interstitial fibrosis.Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Transición Epitelial-Mesenquimal
/
Enfermedades Renales
Límite:
Humans
Idioma:
En
Revista:
Tissue Cell
Año:
2022
Tipo del documento:
Article
País de afiliación:
China