Circadian protein CLOCK modulates regulatory B cell functions of nurses engaging day-night shift rotation.
Cell Signal
; 96: 110362, 2022 08.
Article
en En
| MEDLINE
| ID: mdl-35644424
ABSTRACT
Circadian rhythm proteins participate in regulating multiple physiological activities, including immune responses. The day-night shift rotation (DNSR) affects the circadian rhythm. The influence of circadian rhythm disturbance associated with DNSR on the regulatory functions of B cells remains to be studied. In this study, Blood samples were collected from 30 nurses engaging DNSR. The B cells were isolated from blood samples through magnetic cell separation. The regulatory function of IL-10 B cells (B10 cells) was evaluated using immunological assays. The results showed that the IL-10 expression was significantly reduced in B10 cells in nurses after DNSR. The capacity of inducing type 1 regulatory T cells (Tr1 cells) in B10 cells was down regulated. The circadian locomotor output cycles kaput (CLOCK) was increased in B10 cells, which was negatively correlated with the reduction of IL-10 expression in B10 cells. CLOCK formed a complex with c-Maf inducing protein (CMIP) to induce CMIP degradation; this restricted the IL10 gene transcription in B10 cells. B10 cells collected from nurses after DNSR were ineffective in suppressing T-cell proliferation and inducing Tr1 cells. In summary, DNSR affects the immune regulating function of B10 cells by disturbing the circadian rhythm.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Ritmo Circadiano
/
Proteínas CLOCK
/
Linfocitos B Reguladores
Límite:
Humans
Idioma:
En
Revista:
Cell Signal
Año:
2022
Tipo del documento:
Article
País de afiliación:
China