Nanozyme-based sensitive ratiometric fluorescence detection platform for glucose.
Anal Chim Acta
; 1216: 339993, 2022 Jul 11.
Article
en En
| MEDLINE
| ID: mdl-35691675
ABSTRACT
Development and application of carbon-based nanozymes are attracting wide interest in recent years. Abnormal glucose level can be threat to human health. Sensitive and accurate sensing methods for glucose are still of great urgency. In this work, we constructed a sensitive nanozyme-based ratiometric fluorescence sensing platform for glucose. The sensing system composed of copper-doped carbon-based nanozyme (CuAA) with superb peroxidase-like activity and Mg/N doped carbon quantum dots (Mg-N-CQDs) with distinguished fluorescence property. Efficient tandem catalysis of glucose oxidase (Glu Ox) and CuAA, and inner-filter effect (IFE) between 2, 3-diaminophenazine (DAP) and Mg-N-CQDs played crucial roles in this sensing system. The oxidization of glucose was catalyzed by Glu Ox firstly to produce H2O2. In the presence of H2O2, fluorescent DAP was formed from non-fluorescent substrate o-phenylenediamine (OPD) with assistance of CuAA, resulting in generation of emission at 558 nm. Meanwhile, the emission at 444 nm from Mg-N-CQDs was quenched efficiently by DAP through IFE. The ratiometric fluorescence signal I558/I444 increased linearly with glucose concentration in the scope of 2-400 µmol L-1, the limit of detection (LOD) was 1.56 µmol L-1. It was also practicable to apply the method to the determination of glucose in human serum, satisfactory recoveries and RSDs were acquired.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Puntos Cuánticos
/
Glucosa
Tipo de estudio:
Diagnostic_studies
Límite:
Humans
Idioma:
En
Revista:
Anal Chim Acta
Año:
2022
Tipo del documento:
Article