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A novel 7­hypoxia­related long non­coding RNA signature associated with prognosis and proliferation in melanoma.
Luo, Yi; Li, Tinghao; Zhao, Hengguang; Chen, Aijun.
Afiliación
  • Luo Y; Department of Dermatology, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, P.R. China.
  • Li T; Department of Urology, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, P.R. China.
  • Zhao H; Department of Dermatology, The Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, P.R. China.
  • Chen A; Department of Dermatology, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, P.R. China.
Mol Med Rep ; 26(2)2022 Aug.
Article en En | MEDLINE | ID: mdl-35703357
Hypoxia­related long non­coding RNAs (lncRNAs) are important indicators of the poor prognosis of cancers. The present study aimed to explore the potential relationship between melanoma and hypoxia­related lncRNAs. The transcriptome and clinical data of patients with melanoma were downloaded from The Cancer Genome Atlas database. The prognostic hypoxia­related lncRNAs were screened out using Pearson's correlation test and univariate Cox analysis. As a result, a hypoxia­related­lncRNA signature based on the expression of 7 lncRNAs was constructed, with one unfavourable [MIR205 host gene (MIR205HG)] and six favourable (T cell receptor ß variable 11­2, HLA­DQB1 antisense RNA 1, AL365361.1, AC004847.1, ubiquitin specific peptidase 30 antisense RNA 1 and AC022706.1) lncRNAs as prognostic factors for melanoma. Patients with melanoma were divided into high­ and low­risk groups based on the risk score obtained. Survival analyses were performed to assess the prognostic value of the present risk model. Potential tumour­associated biological pathways associated with the present signature were explored using gene set enrichment analysis. The CIBERSORT algorithm demonstrated the important role of the hypoxia­related lncRNAs in regulating tumour­infiltrating immune cells. Clinical samples collected from our center partly confirmed our findings. Cell Counting Kit­8 and flow cytometry assays indicated the suppression of proliferation of melanoma cells following inhibition of MIR205HG expression. Indicators of the canonical Wnt/ß­catenin signalling pathway were detected by western blotting. The present study demonstrated that MIR205HG could promote melanoma cell proliferation partly via the canonical Wnt/ß­catenin signalling pathway. These findings indicated a 7­hypoxia­related­lncRNA signature that can serve as a novel predictor of melanoma prognosis.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: MicroARNs / ARN Largo no Codificante / Melanoma Tipo de estudio: Prognostic_studies / Risk_factors_studies Límite: Humans Idioma: En Revista: Mol Med Rep Año: 2022 Tipo del documento: Article Pais de publicación: Grecia

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: MicroARNs / ARN Largo no Codificante / Melanoma Tipo de estudio: Prognostic_studies / Risk_factors_studies Límite: Humans Idioma: En Revista: Mol Med Rep Año: 2022 Tipo del documento: Article Pais de publicación: Grecia