Validation of a Novel Double Control Quantitative Copy Number PCR Method to Quantify Off-Target Transgene Integration after CRISPR-Induced DNA Modification.

Schjeide, Brit-Maren Michaud; Schenke, Maren; Seeger, Bettina; Püschel, Gerhard Paul

Schjeide, Brit-Maren Michaud; Schenke, Maren; Seeger, Bettina; Püschel, Gerhard Paul.

Afiliación

Schjeide BM; Department of Nutritional Biochemistry, Institute of Nutritional Science, University of Potsdam, 14558 Nuthetal, Germany.
Schenke M; Department of Nutritional Biochemistry, Faculty of Life Sciences: Food, Nutrition and Health, University of Bayreuth, 95326 Kulmbach, Germany.
Seeger B; Research Group Food Toxicology and Alternative/Complementary Methods to Animal Experiments, Institute for Food Quality and Safety, University of Veterinary Medicine Hannover, 30173 Hannover, Germany.
Püschel GP; Research Group Food Toxicology and Alternative/Complementary Methods to Animal Experiments, Institute for Food Quality and Safety, University of Veterinary Medicine Hannover, 30173 Hannover, Germany.

Methods Protoc ; 5(3)2022 May 25.

Article en En | MEDLINE | ID: mdl-35736544

Palabras clave

CRISPR editing validation; copy number analyses; homologous recombination deficiency; homology-directed repair

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Methods Protoc Año: 2022 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Suiza

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