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Aerosol-jet-printed graphene electrochemical immunosensors for rapid and label-free detection of SARS-CoV-2 in saliva.
Pola, Cícero C; Rangnekar, Sonal V; Sheets, Robert; Szydlowska, Beata M; Downing, Julia R; Parate, Kshama W; Wallace, Shay G; Tsai, Daphne; Hersam, Mark C; Gomes, Carmen L; Claussen, Jonathan C.
Afiliación
  • Pola CC; Department of Mechanical Engineering, Iowa State University, Ames, IA 50011, USA.
  • Rangnekar SV; Department of Materials Science and Engineering, Northwestern University, Evanston, IL 60208, USA.
  • Sheets R; Department of Mechanical Engineering, Iowa State University, Ames, IA 50011, USA.
  • Szydlowska BM; Department of Materials Science and Engineering, Northwestern University, Evanston, IL 60208, USA.
  • Downing JR; Department of Materials Science and Engineering, Northwestern University, Evanston, IL 60208, USA.
  • Parate KW; Department of Mechanical Engineering, Iowa State University, Ames, IA 50011, USA.
  • Wallace SG; Department of Materials Science and Engineering, Northwestern University, Evanston, IL 60208, USA.
  • Tsai D; Department of Materials Science and Engineering, Northwestern University, Evanston, IL 60208, USA.
  • Hersam MC; Department of Materials Science and Engineering, Northwestern University, Evanston, IL 60208, USA.
  • Gomes CL; Department of Chemistry, Northwestern University, Evanston, IL 60208, USA.
  • Claussen JC; Department of Electrical and Computer Engineering, Northwestern University, Evanston, IL 60208, USA.
2d Mater ; 9(3)2022 Jul.
Article en En | MEDLINE | ID: mdl-35785019
ABSTRACT
Rapid, inexpensive, and easy-to-use coronavirus disease 2019 (COVID-19) home tests are key tools in addition to vaccines in the world-wide fight to eliminate national and local shutdowns. However, currently available tests for SARS-CoV-2, the virus that causes COVID-19, are too expensive, painful, and irritating, or not sufficiently sensitive for routine, accurate home testing. Herein, we employ custom-formulated graphene inks and aerosol jet printing (AJP) to create a rapid electrochemical immunosensor for direct detection of SARS-CoV-2 Spike Receptor-Binding Domain (RBD) in saliva samples acquired non-invasively. This sensor demonstrated limits of detection that are considerably lower than most commercial SARS-CoV-2 antigen tests (22.91 ± 4.72 pg/mL for Spike RBD and 110.38 ± 9.00 pg/mL for Spike S1) as well as fast response time (~30 mins), which was facilitated by the functionalization of printed graphene electrodes in a single-step with SARS-CoV-2 polyclonal antibody through the carbodiimide reaction without the need for nanoparticle functionalization or secondary antibody or metallic nanoparticle labels. This immunosensor presents a wide linear sensing range from 1 to 1000 ng/mL and does not react with other coexisting influenza viruses such as H1N1 hemagglutinin. By combining high-yield graphene ink synthesis, automated printing, high antigen selectivity, and rapid testing capability, this work offers a promising alternative to current SARS-CoV-2 antigen tests.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Diagnostic_studies Idioma: En Revista: 2d Mater Año: 2022 Tipo del documento: Article País de afiliación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Diagnostic_studies Idioma: En Revista: 2d Mater Año: 2022 Tipo del documento: Article País de afiliación: Estados Unidos