Lightsheet Microscopy.
Curr Protoc
; 2(7): e448, 2022 Jul.
Article
en En
| MEDLINE
| ID: mdl-35838628
In this paper, we review lightsheet (selective plane illumination) microscopy for mouse developmental biologists. There are different means of forming the illumination sheet, and we discuss these. We explain how we introduced the lightsheet microscope economically into our core facility and present our results on fixed and living samples. We also describe methods of clearing fixed samples for three-dimensional imaging and discuss the various means of preparing samples with particular reference to mouse cilia, adipose spheroids, and cochleae. © 2022 The Authors. Current Protocols published by Wiley Periodicals LLC.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Iluminación
/
Imagenología Tridimensional
Límite:
Animals
Idioma:
En
Revista:
Curr Protoc
Año:
2022
Tipo del documento:
Article
Pais de publicación:
Estados Unidos