Alginate-based hydrogel fiber as a restricted access material for microextraction of drugs in biological samples.
Carbohydr Polym
; 294: 119810, 2022 Oct 15.
Article
en En
| MEDLINE
| ID: mdl-35868799
ABSTRACT
A novel lab-made alginate-based hydrogel device was successfully prepared and applied as a sorption material for the solid-phase microextraction of drugs (fluoxetine and its metabolite, norfluoxetine) in human plasma, with subsequent determination by high performance liquid chromatography-fluorescence detection (HPLC-FD). When supported in a polypropylene hollow fiber, the alginate was able to extract the analytes and functioned as a restricted access material, excluding >95 % of proteins from the biological matrix. The results indicate the potential use of this phase/device for quantitative drugs extraction from biological matrices at concentrations compatible with those typical in the literature (0.5 µg mL-1), and with satisfactory precision (13.4 % for fluoxetine and 6.2 % for norfluoxetine). Such outcomes, promoted by a simple and inexpensive material, open a new perspective of exploration of hydrogels as the sorption phase in biological matrices, a concept previously unexplored in the literature.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Fluoxetina
/
Hidrogeles
Límite:
Humans
Idioma:
En
Revista:
Carbohydr Polym
Año:
2022
Tipo del documento:
Article
País de afiliación:
Brasil