New mode of liquid chromatography separation: A new mode of separation using multi-charged ions in the mobile phase for high-performance liquid chromatography.

ABSTRACT

In liquid chromatography, several types of separation modes are known. Here, we are going to describe a new mode of retention and consecutively, a separation mechanism that to our knowledge was not identified as such before. We named this mode Bridge Ion Separation Technique. This separation mode is possible when a double-charged ionic modifier of the mobile phase forms a dynamic bridge between an identically charged stationary phase and analyte. This phenomenon was observed in the water-reduced mobile phase and it is universal for both anions and cations as long as the column has the same charge as the analytes. This retention mechanism is observed with any number of charged functional groups in the analyte molecule but is more profound when the number of charges in the analyte molecule is increased.