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Complete-genome sequence and in vitro probiotic characteristics analysis of Bifidobacterium pseudolongum YY-26.
Yu, Ying; Ren, Xinmiao; Cao, Linyuan; Liang, Qingping; Xiao, Mengshi; Cheng, Jiaying; Nan, Shihao; Zhu, Changliang; Kong, Qing; Fu, Xiaodan; Mou, Haijin.
Afiliación
  • Yu Y; College of Food Science and Engineering, Ocean University of China, Qingdao, China.
  • Ren X; College of Food Science and Engineering, Ocean University of China, Qingdao, China.
  • Cao L; College of Food Science and Engineering, Ocean University of China, Qingdao, China.
  • Liang Q; College of Food Science and Engineering, Ocean University of China, Qingdao, China.
  • Xiao M; College of Food Science and Engineering, Ocean University of China, Qingdao, China.
  • Cheng J; College of Food Science and Engineering, Ocean University of China, Qingdao, China.
  • Nan S; State Key Laboratory of Food Science and Technology, China-Canada Joint Laboratory of Food Science and Technology (Nanchang), Key Laboratory of Bioactive Polysaccharides of Jiangxi Province, Nanchang University, Nanchang, China.
  • Zhu C; College of Food Science and Engineering, Ocean University of China, Qingdao, China.
  • Kong Q; College of Food Science and Engineering, Ocean University of China, Qingdao, China.
  • Fu X; State Key Laboratory of Food Science and Technology, China-Canada Joint Laboratory of Food Science and Technology (Nanchang), Key Laboratory of Bioactive Polysaccharides of Jiangxi Province, Nanchang University, Nanchang, China.
  • Mou H; College of Food Science and Engineering, Ocean University of China, Qingdao, China.
J Appl Microbiol ; 133(4): 2599-2617, 2022 Oct.
Article en En | MEDLINE | ID: mdl-35870146
ABSTRACT

AIMS:

The aim was to isolate a neotype bifidobacteria strain and evaluate its in vitro probiotic potential. METHODS AND

RESULTS:

Bifidobacterium pseudolongum YY-26 (CGMCC 24310) was isolated from faeces of mice treated with low-molecular-weight hydrolyzed guar gum (GMPS) and identified based on 16S rRNA sequence and genome sequence. Whole-genome sequencing obtained using PacBio's single-molecular and Illumina's paired-end sequencing technology. A genome of 2.1 Mb in length, with 1877 predicted protein-coding sequences was obtained. Carbohydrate-Activity enZyme analysis revealed that YY-26 encodes 66 enzymes related to carbohydrate metabolism. Whole genome sequence analysis revealed the typical probiotic characteristics of YY-26, including safety in genetic level and ability to produce beneficial metabolites and extracellular polysaccharides. Ability of extensive carbon source utilization and short-chain fatty acid production was observed with single YY-26 cultivation. Considerable acetic acids and lactic acids were determined in GMPS utilization. YY-26 showed tolerance to simulated gastrointestinal tract and displayed appreciable antioxidant activity of free radical scavenging.

CONCLUSIONS:

B. pseudolongum YY-26 was identified with numerous probiotic-associated genes and its probiotic characteristics were verified in vitro. SIGNIFICANCE AND IMPACT OF STUDY This study supplemented with limited publicly information regarding the genomes of B. pseudolongum strains and revealed the probiotic potential of YY-26.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Probióticos / Antioxidantes Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: J Appl Microbiol Asunto de la revista: MICROBIOLOGIA Año: 2022 Tipo del documento: Article País de afiliación: China
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Probióticos / Antioxidantes Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: J Appl Microbiol Asunto de la revista: MICROBIOLOGIA Año: 2022 Tipo del documento: Article País de afiliación: China