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In situ detection of protein-protein interaction by proximity ligation assay in patient derived brain tumor stem cells.
Sharanek, Ahmad; Raco, Laura; Soleimani, Vahab D; Jahani-Asl, Arezu.
Afiliación
  • Sharanek A; Department of Cellular and Molecular Medicine and University of Ottawa Brain and Mind Research Institute, University of Ottawa, 451 Smyth Road, Ottawa, ON K1H 8M5, Canada; Lady Davis Institute for Medical Research, Jewish General Hospital, Chemin de la Côte-Sainte-Catherine, Montréal, QC H3T 1E2, Ca
  • Raco L; Lady Davis Institute for Medical Research, Jewish General Hospital, Chemin de la Côte-Sainte-Catherine, Montréal, QC H3T 1E2, Canada; Division of Experimental Medicine, McGill University, 1001 Decarie Boulevard, Montreal, QC H4A 3J1, Canada.
  • Soleimani VD; Lady Davis Institute for Medical Research, Jewish General Hospital, Chemin de la Côte-Sainte-Catherine, Montréal, QC H3T 1E2, Canada; Division of Experimental Medicine, McGill University, 1001 Decarie Boulevard, Montreal, QC H4A 3J1, Canada; Department of Human Genetics, McGill University, 3640 Rue
  • Jahani-Asl A; Department of Cellular and Molecular Medicine and University of Ottawa Brain and Mind Research Institute, University of Ottawa, 451 Smyth Road, Ottawa, ON K1H 8M5, Canada; Division of Experimental Medicine, McGill University, 1001 Decarie Boulevard, Montreal, QC H4A 3J1, Canada. Electronic address:
STAR Protoc ; 3(3): 101554, 2022 09 16.
Article en En | MEDLINE | ID: mdl-35880130
ABSTRACT
Improper or aberrant protein-protein interactions can lead to severe human diseases including cancer. Here, we describe an adapted proximity ligation assay (PLA) protocol for the assessment of galectin-1-HOXA5 interaction in brain tumor stem cells (BTSCs). We detail the steps for culturing and preparation of BTSCs followed by PLA and detection of protein interactions in situ using fluorescent microscopy. This PLA protocol is optimized specifically for BTSCs and includes key controls for effective result analysis. For complete details on the use and execution of this protocol, please refer to Sharanek et al. (2021).
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Encéfalo / Mapeo de Interacción de Proteínas Tipo de estudio: Diagnostic_studies Límite: Humans Idioma: En Revista: STAR Protoc Año: 2022 Tipo del documento: Article País de afiliación: Canadá
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Encéfalo / Mapeo de Interacción de Proteínas Tipo de estudio: Diagnostic_studies Límite: Humans Idioma: En Revista: STAR Protoc Año: 2022 Tipo del documento: Article País de afiliación: Canadá