The expression of Phase II drug-metabolizing enzymes in human B-lymphoblastoid TK6 cells.
J Environ Sci Health C Toxicol Carcinog
; 40(1): 106-118, 2022.
Article
en En
| MEDLINE
| ID: mdl-35895929
ABSTRACT
In vitro genotoxicity testing plays an important role in chemical risk assessment. The human B-lymphoblastoid cell line TK6 is widely used as a standard cell line for regulatory safety evaluations. Like many other mammalian cell lines, TK6 cells have limited metabolic capacity; therefore, usually require a source of exogenous metabolic activation for use in genotoxicity testing. Previously, we developed a set of TK6-derived cell lines that individually express one of fourteen cytochrome P450s (CYPs). In the present study, we surveyed a panel of major Phase II drug-metabolizing enzymes to characterize their baseline expression in TK6 cells. These results may serve as a reference enzymatic profile of this commonly used cell line.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Daño del ADN
/
Mamíferos
Tipo de estudio:
Risk_factors_studies
Límite:
Animals
/
Humans
Idioma:
En
Revista:
J Environ Sci Health C Toxicol Carcinog
Año:
2022
Tipo del documento:
Article
País de afiliación:
Estados Unidos