Study of a haploid yeast strain with an unusually high rDNA content. III. Unequal meiotic segregation of the gamma-DNA fraction.
Biochim Biophys Acta
; 520(1): 88-102, 1978 Aug 23.
Article
en En
| MEDLINE
| ID: mdl-359052
ABSTRACT
DNA from different strains of Saccharomyces cerevisiae has been fractionated in preparative Ag+/Cs2-SO4 density gradients. The results show that there are real differences in amount of the nuclear satellite component, the gamma-DNA, from one strain to the other. The gamma-DNA forms a homogeneous dense band that contains all the rDNA, and the amount of gamma-DNA estimated from the gradients can be correlated to amount of rDNA derived from rRNA-DNA hybridizations. By various crossings and sporulations we have obtained diploid and haploid strains with gamma-DNA contents ranging from 7 to 20% of the nuclear DNA. During meiosis, the amount of gamma-DNA appears to segregate in a pattern that indicates unequal crossing over as a possible mechanism for differences in gamma-DNA contents.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Ribosomas
/
Saccharomyces cerevisiae
/
ADN
/
ADN Satélite
Idioma:
En
Revista:
Biochim Biophys Acta
Año:
1978
Tipo del documento:
Article