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Robust counterselection and advanced λRed recombineering enable markerless chromosomal integration of large heterologous constructs.
Bubnov, Dmitrii M; Yuzbashev, Tigran V; Khozov, Andrey A; Melkina, Olga E; Vybornaya, Tatiana V; Stan, Guy-Bart; Sineoky, Sergey P.
Afiliación
  • Bubnov DM; Bioresource Center Russian National Collection of Industrial Microorganisms (BRC VKPM), State Research Institute for Genetics and Selection of Industrial Microorganisms of National Research Center 'Kurchatov Institute' (NRC 'Kurchatov Institute' - GosNIIgenetika), 1-st Dorozhny pr., 1, Moscow 117545
  • Yuzbashev TV; Kurchatov Complex of Genetic Research, NRC 'Kurchatov Institute', Kurchatov Square, 1, Moscow 123098, Russia.
  • Khozov AA; Department of Bioengineering and Imperial College Centre for Synthetic Biology, Imperial College London, London SW7 2AZ, UK.
  • Melkina OE; Bioresource Center Russian National Collection of Industrial Microorganisms (BRC VKPM), State Research Institute for Genetics and Selection of Industrial Microorganisms of National Research Center 'Kurchatov Institute' (NRC 'Kurchatov Institute' - GosNIIgenetika), 1-st Dorozhny pr., 1, Moscow 117545
  • Vybornaya TV; Kurchatov Complex of Genetic Research, NRC 'Kurchatov Institute', Kurchatov Square, 1, Moscow 123098, Russia.
  • Stan GB; Department of Microbiology, Faculty of Biology, Lomonosov Moscow State University, Lenin's Hills 1-12, Moscow 119234, Russia.
  • Sineoky SP; Kurchatov Complex of Genetic Research, NRC 'Kurchatov Institute', Kurchatov Square, 1, Moscow 123098, Russia.
Nucleic Acids Res ; 50(15): 8947-8960, 2022 08 26.
Article en En | MEDLINE | ID: mdl-35920321
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Bacteriófago lambda / Escherichia coli Idioma: En Revista: Nucleic Acids Res Año: 2022 Tipo del documento: Article Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Bacteriófago lambda / Escherichia coli Idioma: En Revista: Nucleic Acids Res Año: 2022 Tipo del documento: Article Pais de publicación: Reino Unido