Your browser doesn't support javascript.
loading
Atomic resolution Cryo-EM structure of human proteasome activator PA28γ.
Chen, Dan-Dan; Hao, Jia; Shen, Chao-Hui; Deng, Xian-Ming; Yun, Cai-Hong.
Afiliación
  • Chen DD; Department of Biochemistry and Biophysics, School of Basic Medical Sciences, Peking University, Beijing 100191, China.
  • Hao J; Hongyun Biotech Co., Ltd., Nanjing, Jiangsu 211112, China.
  • Shen CH; National Key Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, PR China.
  • Deng XM; State Key Laboratory of Cellular Stress Biology, Innovation Center for Cell Signaling Network, School of Life Sciences, Xiamen University, Xiamen, Fujian 361102, China.
  • Yun CH; Department of Biochemistry and Biophysics, School of Basic Medical Sciences, Peking University, Beijing 100191, China. Electronic address: yunch@pku.edu.cn.
Int J Biol Macromol ; 219: 500-507, 2022 Oct 31.
Article en En | MEDLINE | ID: mdl-35932807
The PA28 family proteasome activators play important roles in regulating proteasome activities. Though the three paralogs (PA28α, PA28ß, and PA28γ) are similar in terms of primary sequence, they show significant differences in expression pattern, cellular localization and most importantly, biological functions. While PA28αß is responsible for promoting peptidase activity of proteasome to facilitate MHC-I antigen processing, but unable to promote protein degradation, PA28γ is well-known to not only promote peptidase activity but also proteolytic activity of proteasome. However, why this paralog has the unique function remains elusive. Previous structural studies have mainly focused on mammalian PA28α, PA28ß and PA28αß heptamers, while structural studies on mammalian PA28γ of atomic resolution are still absent to date. In the present work, we determined the Cryo-EM structure of the human PA28γ heptamer at atomic resolution, revealing interesting unique structural features that may hint our understanding the functional mechanisms of this proteasome activator.
Asunto(s)
Palabras clave

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Autoantígenos / Complejo de la Endopetidasa Proteasomal Límite: Animals / Humans Idioma: En Revista: Int J Biol Macromol Año: 2022 Tipo del documento: Article País de afiliación: China Pais de publicación: Países Bajos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Autoantígenos / Complejo de la Endopetidasa Proteasomal Límite: Animals / Humans Idioma: En Revista: Int J Biol Macromol Año: 2022 Tipo del documento: Article País de afiliación: China Pais de publicación: Países Bajos