A new metal-free benzorhodol-based photoluminophore selective for carbon monoxide detection applicable in both in vitro and in vivo bioimaging.
Anal Methods
; 14(33): 3196-3202, 2022 08 25.
Article
en En
| MEDLINE
| ID: mdl-35938936
ABSTRACT
A new benzorhodol-based non-fluorescent organic frame (DEB-CO) detects carbon monoxide (CO) selectively through a spirolactam ring-opening mechanism. Herein, the selective off-on fluorogenic behavior of this probe towards CO has been achieved without any assistance of precious and hazardous metals (e.g. Pd2+) as additional substrates. Moreover, the red-emissive probe motivated us to apply in situ tracing in mice and living cells. The selective off-on fluorogenic behavior of this probe towards CO originating from CORM-3 in vitro and in vivo with a limit of detection as low as 64.29 nM (for CORM-3) has been observed. Additionally, this probe is capable of sensing toxic carbon monoxide gas. This probe has also been utilized to detect intracellular CO in MCF7 cells (in vitro) and to spot the distribution of CO in mice (in vivo) by acquiring bioimages with the help of confocal microscopy, which indicates that DEB-CO is a smart competent probe for this purpose.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Monóxido de Carbono
/
Colorantes Fluorescentes
Tipo de estudio:
Diagnostic_studies
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Anal Methods
Año:
2022
Tipo del documento:
Article
País de afiliación:
India