Your browser doesn't support javascript.
loading
Islet Gene View-a tool to facilitate islet research.
Asplund, Olof; Storm, Petter; Chandra, Vikash; Hatem, Gad; Ottosson-Laakso, Emilia; Mansour-Aly, Dina; Krus, Ulrika; Ibrahim, Hazem; Ahlqvist, Emma; Tuomi, Tiinamaija; Renström, Erik; Korsgren, Olle; Wierup, Nils; Ibberson, Mark; Solimena, Michele; Marchetti, Piero; Wollheim, Claes; Artner, Isabella; Mulder, Hindrik; Hansson, Ola; Otonkoski, Timo; Groop, Leif; Prasad, Rashmi B.
Afiliación
  • Asplund O; Department of Clinical Sciences, Clinical Research Centre, Lund University, Malmö, Sweden.
  • Storm P; Lund University Diabetes Centre (LUDC), Lund, Sweden.
  • Chandra V; Department of Clinical Sciences, Clinical Research Centre, Lund University, Malmö, Sweden.
  • Hatem G; Lund University Diabetes Centre (LUDC), Lund, Sweden.
  • Ottosson-Laakso E; Department of Experimental Medical Science, Developmental and Regenerative Neurobiology, Wallenberg Neuroscience Center, Lund, Sweden.
  • Mansour-Aly D; Stem Cells and Metabolism Research Program, Faculty of Medicine, University of Helsinki, Helsinki, Finland.
  • Krus U; Department of Clinical Sciences, Clinical Research Centre, Lund University, Malmö, Sweden.
  • Ibrahim H; Lund University Diabetes Centre (LUDC), Lund, Sweden.
  • Ahlqvist E; Department of Clinical Sciences, Clinical Research Centre, Lund University, Malmö, Sweden.
  • Tuomi T; Lund University Diabetes Centre (LUDC), Lund, Sweden.
  • Renström E; Department of Clinical Sciences, Clinical Research Centre, Lund University, Malmö, Sweden.
  • Korsgren O; Lund University Diabetes Centre (LUDC), Lund, Sweden.
  • Wierup N; Department of Clinical Sciences, Clinical Research Centre, Lund University, Malmö, Sweden.
  • Ibberson M; Lund University Diabetes Centre (LUDC), Lund, Sweden.
  • Solimena M; Stem Cells and Metabolism Research Program, Faculty of Medicine, University of Helsinki, Helsinki, Finland.
  • Marchetti P; Department of Clinical Sciences, Clinical Research Centre, Lund University, Malmö, Sweden.
  • Wollheim C; Lund University Diabetes Centre (LUDC), Lund, Sweden.
  • Artner I; Department of Clinical Sciences, Clinical Research Centre, Lund University, Malmö, Sweden.
  • Mulder H; Lund University Diabetes Centre (LUDC), Lund, Sweden.
  • Hansson O; Department of Endocrinology, Abdominal Centre, Helsinki University Hospital, Folkhalsan Research Center, Helsinki, Finland.
  • Otonkoski T; Institute for Molecular Medicine Finland (FIMM), University of Helsinki, Helsinki, Finland.
  • Groop L; Department of Clinical Sciences, Clinical Research Centre, Lund University, Malmö, Sweden.
  • Prasad RB; Lund University Diabetes Centre (LUDC), Lund, Sweden.
Life Sci Alliance ; 5(12)2022 08 10.
Article en En | MEDLINE | ID: mdl-35948367
Characterization of gene expression in pancreatic islets and its alteration in type 2 diabetes (T2D) are vital in understanding islet function and T2D pathogenesis. We leveraged RNA sequencing and genome-wide genotyping in islets from 188 donors to create the Islet Gene View (IGW) platform to make this information easily accessible to the scientific community. Expression data were related to islet phenotypes, diabetes status, other islet-expressed genes, islet hormone-encoding genes and for expression in insulin target tissues. The IGW web application produces output graphs for a particular gene of interest. In IGW, 284 differentially expressed genes (DEGs) were identified in T2D donor islets compared with controls. Forty percent of DEGs showed cell-type enrichment and a large proportion significantly co-expressed with islet hormone-encoding genes; glucagon (<i>GCG</i>, 56%), amylin (<i>IAPP</i>, 52%), insulin (<i>INS</i>, 44%), and somatostatin (<i>SST</i>, 24%). Inhibition of two DEGs, <i>UNC5D</i> and <i>SERPINE2</i>, impaired glucose-stimulated insulin secretion and impacted cell survival in a human ß-cell model. The exploratory use of IGW could help designing more comprehensive functional follow-up studies and serve to identify therapeutic targets in T2D.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Islotes Pancreáticos / Diabetes Mellitus Tipo 2 Tipo de estudio: Observational_studies / Prognostic_studies Límite: Humans Idioma: En Revista: Life Sci Alliance Año: 2022 Tipo del documento: Article País de afiliación: Suecia Pais de publicación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Islotes Pancreáticos / Diabetes Mellitus Tipo 2 Tipo de estudio: Observational_studies / Prognostic_studies Límite: Humans Idioma: En Revista: Life Sci Alliance Año: 2022 Tipo del documento: Article País de afiliación: Suecia Pais de publicación: Estados Unidos