Left Coronary Artery Ligation: A Surgical Murine Model of Myocardial Infarction.
J Vis Exp
; (186)2022 08 09.
Article
en En
| MEDLINE
| ID: mdl-36036590
ABSTRACT
Ischemic heart disease and subsequent myocardial infarction (MI) is one of the leading causes of mortality in the United States and around the world. In order to explore the pathophysiological changes after myocardial infarction and design future treatments, research models of MI are required. Permanent ligation of the left coronary artery (LCA) in mice is a popular model to investigate cardiac function and ventricular remodeling post MI. Here we describe a less invasive, reliable, and reproducible surgical murine MI model by permanent ligation of the LCA. Our surgical model comprises of an easily reversible general anesthesia, endotracheal intubation that does not require a tracheotomy, and a thoracotomy. Electrocardiography and troponin measurement should be performed to ensure MI. Echocardiography at day 28 after MI will discern heart function and heart failure parameters. The degree of cardiac fibrosis can be evaluated by Masson's trichrome staining and cardiac MRI. This MI model is useful for studying the pathophysiological and immunological alterations after MI.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Vasos Coronarios
/
Infarto del Miocardio
Tipo de estudio:
Etiology_studies
Límite:
Animals
Idioma:
En
Revista:
J Vis Exp
Año:
2022
Tipo del documento:
Article