Kinetics of lipid radical formation in lipoproteins from ß-thalassemia: Implication of cholesteryl esters and α-tocopherol.
Biomed Pharmacother
; 154: 113624, 2022 Oct.
Article
en En
| MEDLINE
| ID: mdl-36057220
ABSTRACT
Vascular complications in ß-thalassemia are associated with oxidative modification of lipoproteins under high oxidative stress. The lipid components of lipoproteins are oxidized via lipid peroxidation and produce lipid radicals (Lâ¢) as the key initial intermediates. Modification of lipid components, therefore, might result in alterations in the rate and products of lipid peroxidation. In this study, the kinetics of L⢠formation during the 2,2'-Azobis(2-amidinopropane) dihydrochloride (AAPH)- and hemin-induced oxidation of low-density and high-density lipoproteins (LDL and HDL) from ß-thalassemia patients and healthy volunteers were investigated using a specific and sensitive fluorescence probe for Lâ¢. Kinetic parameters, including initial lag time, propagation rate and total L⢠production, were calculated by monitoring a fluorescence-active NBD-Pen-L⢠adduct. Oxidation of thalassemia lipoproteins exhibited a significantly shorter lag time but a slower propagation rate of L⢠formation when compared with healthy lipoproteins. LDL showed higher resistance to oxidation during the initiation phase but higher L⢠formation than HDL. Our results indicated that the levels of α-tocopherol determined the initial lag time, whereas the levels of core lipids and cholesteryl esters, especially cholesteryl linoleate (CL), determined the propagation rate and total L⢠production. The difference in potency of AAPH and hemin supported that hemin preferentially targeted core lipids. Moreover, analysis of 13-hydroxyoctadecadienoic acid cholesteryl ester (13-HODE-CE)/CE ratio indicated that thalassemia lipoproteins have higher susceptibility to oxidation than healthy lipoproteins. In conclusion, our findings suggested that CL and α-tocopherol were implicated in the susceptibility of lipoproteins to lipid peroxidation in physiological and pathological conditions of ß-thalassemia.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Talasemia beta
/
Alfa-Tocoferol
Límite:
Humans
Idioma:
En
Revista:
Biomed Pharmacother
Año:
2022
Tipo del documento:
Article