Single-Cell Profiling of the Intrinsic Apoptotic Pathway by Mass Cytometry (CyTOF).
Methods Mol Biol
; 2543: 83-97, 2022.
Article
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| MEDLINE
| ID: mdl-36087261
ABSTRACT
Mass cytometry time-of-flight (CyTOF) is a technology for the study of complex biological processes at the single-cell level. The technology enables measurement of >50 protein moieties on the surface and inside the cell. The power of CyTOF lies in the application of purpose-built panels of antibody probes that resolve features of key biological processes in a cell. Here, we describe this technology's use to profile changes in the intrinsic apoptotic (cell death) protein machinery at a single-cell level. We provide a comprehensive overview of a tailor-made set of cell survival/death antibodies, ideal staining conditions, and high-dimensional data analysis.
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01-internacional
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MEDLINE
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En
Revista:
Methods Mol Biol
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2022
Tipo del documento:
Article
País de afiliación:
Australia