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Municipal and neighbourhood level wastewater surveillance and subtyping of an influenza virus outbreak.
Mercier, Elisabeth; D'Aoust, Patrick M; Thakali, Ocean; Hegazy, Nada; Jia, Jian-Jun; Zhang, Zhihao; Eid, Walaa; Plaza-Diaz, Julio; Kabir, Md Pervez; Fang, Wanting; Cowan, Aaron; Stephenson, Sean E; Pisharody, Lakshmi; MacKenzie, Alex E; Graber, Tyson E; Wan, Shen; Delatolla, Robert.
Afiliación
  • Mercier E; Department of Civil Engineering, University of Ottawa, Ottawa, K1N 6N5, Canada.
  • D'Aoust PM; Department of Civil Engineering, University of Ottawa, Ottawa, K1N 6N5, Canada.
  • Thakali O; Department of Civil Engineering, University of Ottawa, Ottawa, K1N 6N5, Canada.
  • Hegazy N; Department of Civil Engineering, University of Ottawa, Ottawa, K1N 6N5, Canada.
  • Jia JJ; Department of Civil Engineering, University of Ottawa, Ottawa, K1N 6N5, Canada.
  • Zhang Z; Department of Civil Engineering, University of Ottawa, Ottawa, K1N 6N5, Canada.
  • Eid W; Children's Hospital of Eastern Ontario Research Institute, Ottawa, K1H 8L1, Canada.
  • Plaza-Diaz J; Children's Hospital of Eastern Ontario Research Institute, Ottawa, K1H 8L1, Canada.
  • Kabir MP; Department of Civil Engineering, University of Ottawa, Ottawa, K1N 6N5, Canada.
  • Fang W; Department of Civil Engineering, University of Ottawa, Ottawa, K1N 6N5, Canada.
  • Cowan A; Department of Civil Engineering, University of Ottawa, Ottawa, K1N 6N5, Canada.
  • Stephenson SE; Children's Hospital of Eastern Ontario Research Institute, Ottawa, K1H 8L1, Canada.
  • Pisharody L; Department of Civil Engineering, University of Ottawa, Ottawa, K1N 6N5, Canada.
  • MacKenzie AE; Children's Hospital of Eastern Ontario Research Institute, Ottawa, K1H 8L1, Canada.
  • Graber TE; Children's Hospital of Eastern Ontario Research Institute, Ottawa, K1H 8L1, Canada.
  • Wan S; Department of Civil Engineering, University of Ottawa, Ottawa, K1N 6N5, Canada.
  • Delatolla R; Department of Civil Engineering, University of Ottawa, Ottawa, K1N 6N5, Canada. robert.delatolla@uOttawa.ca.
Sci Rep ; 12(1): 15777, 2022 09 22.
Article en En | MEDLINE | ID: mdl-36138059
ABSTRACT
Recurrent influenza epidemics and pandemic potential are significant risks to global health. Public health authorities use clinical surveillance to locate and monitor influenza and influenza-like cases and outbreaks to mitigate hospitalizations and deaths. Currently, global integration of clinical surveillance is the only reliable method for reporting influenza types and subtypes to warn of emergent pandemic strains. The utility of wastewater surveillance (WWS) during the COVID-19 pandemic as a less resource intensive replacement or complement for clinical surveillance has been predicated on analyzing viral fragments in wastewater. We show here that influenza virus targets are stable in wastewater and partitions favorably to the solids fraction. By quantifying, typing, and subtyping the virus in municipal wastewater and primary sludge during a community outbreak, we forecasted a citywide flu outbreak with a 17-day lead time and provided population-level viral subtyping in near real-time to show the feasibility of influenza virus WWS at the municipal and neighbourhood levels in near real time using minimal resources and infrastructure.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Gripe Humana / Subtipo H1N1 del Virus de la Influenza A / COVID-19 Tipo de estudio: Screening_studies Límite: Humans Idioma: En Revista: Sci Rep Año: 2022 Tipo del documento: Article País de afiliación: Canadá

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Gripe Humana / Subtipo H1N1 del Virus de la Influenza A / COVID-19 Tipo de estudio: Screening_studies Límite: Humans Idioma: En Revista: Sci Rep Año: 2022 Tipo del documento: Article País de afiliación: Canadá