Short-term warming and N deposition alter the photosynthetic pigments trade-off in leaves of Leymus secalinus growing in different alpine grassland habitats on Qinghai-Tibetan plateau.

ABSTRACT

Warming and N (nitrogen) deposition are the two main driving factors of global change. We examined the effects of increased N deposition (8 kg ha-1 year-1) and warming, as well as their combined effect on the leaf photosynthetic pigments of Leymus secalinus, which is one of the key alpine plants growing in different grassland habitats on Qinghai-Tibetan plateau. In 2014, the experiments were established in 12 plots (2×5m) of three types of habitats including alpine meadow (AM), alpine steppe (AS), and cultivated grassland (CG) with the following treatments CK (control treatment), N (only N deposition), W (only warming), and W&N (warming combined with N deposition). Results showed that the effects of warming and N deposition on photosynthetic pigments of Leymus secalinus varied with different grassland habitat types. In three grassland types, warming led to no significant effects on the total chlorophyll content of L. secalinus, while N deposition alone only significantly enhanced total chlorophyll content in alpine meadow and cultivated grassland. N deposition combined with warming only significantly enhanced total chlorophyll content of L. secalinus in alpine steppe and cultivated grassland. Chla content plays an important role in determining the variation of total chlorophyll content. Chla/Chlb ratio of L. secalinus was more stable in alpine meadow compared with that of L. secalinus in the other two grassland types. Car/Chl ratio of L. secalinus was not prone to be affected by warming and N deposition in all grassland types. Leaf N content was obviously positively correlated with photosynthetic pigments, especially Chla content. Warming and N deposition all affected photosynthetic pigment dynamics and tended to increase Chla by enhancing its weight. Our results highlighted that both warming and N deposition as well as their combination can alter the trade-off of photosynthetic pigments through enhancing the Chla ratio in L. secalinus. In addition, growing habitats should be within consideration when studying alpine plants adaptation mechanism to global change in the future.