Long-Term Brain Organotypic Cultures of the Turquoise Killifish Nothobranchius furzeri.
Cold Spring Harb Protoc
; 2022(12): 624-629, 2022 12 01.
Article
en En
| MEDLINE
| ID: mdl-36167677
ABSTRACT
Organotypic culture is a well-established method for culturing ex vivo tissue samples. The advantages of culturing tissue slices for prolonged time periods ex vivo are numerous and consist primarily of the maintenance of the overall in vivo architecture of the isolated sample, the lack of the ematoencephalic barrier, and the ease of pharmacological treatments and interventions that can be conducted under controlled conditions as in in vitro systems such as cell cultures. Given the extremely short life span of Nothobranchius furzeri and the emergence of aging signs only after a few months of life, it is of particular interest to establish this protocol for N. furzeri as a potential method to study brain aging ex vivo.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Ciprinodontiformes
/
Fundulidae
Tipo de estudio:
Guideline
Límite:
Animals
Idioma:
En
Revista:
Cold Spring Harb Protoc
Año:
2022
Tipo del documento:
Article
País de afiliación:
Italia