The application of genome-wide CRISPR-Cas9 screens to dissect the molecular mechanisms of toxins.
Comput Struct Biotechnol J
; 20: 5076-5084, 2022.
Article
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| MEDLINE
| ID: mdl-36187925
ABSTRACT
Many toxins are life-threatening to both animals and humans. However, specific antidotes are not available for most of those toxins. The molecular mechanisms underlying the toxicology of well-known toxins are not yet fully characterized. Recently, the advance in CRISPR-Cas9 technologies has greatly accelerated the process of revealing the toxic mechanisms of some common toxins on hosts from a genome-wide perspective. The high-throughput CRISPR screen has made it feasible to untangle complicated interactions between a particular toxin and its corresponding targeting tissue(s). In this review, we present an overview of recent advances in molecular dissection of toxins' cytotoxicity by using genome-wide CRISPR screens, summarize the components essential for toxin-specific CRISPR screens, and propose new strategies for future research.
AAPCC, American Association of Poison Control Centers; ABE, Adenine Base Editor; Bacterial toxin; CBE, Cytosine Base Editor; CISRPR; CRISPR, Clustered Regularly Interspaced Short Palindromic Repeats; CRISPR-Cas9 screen; CRISPRa, CRISPR activation; CRISPRi, CRISPR interference; DSB, Double-Strand Break; GOF, Gain-of-function; GeCKO; GeCKO, CRISPR Knockout Pooled Library; Genome-wide; LOF, Loss-of-function; MAGeCK, Model-based Analysis of Genome-wide CRISPR-Cas9 Knockout; Mechanism; Mycotoxin; NGS, Next-Generation Sequencing; NHEJ, Non-Homologous End Joining; Toxicant; Toxin; Venom; sgRNA, single guide RNA
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01-internacional
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MEDLINE
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En
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Comput Struct Biotechnol J
Año:
2022
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Article