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Daphne genkwa flower extract promotes the neuroprotective effects of microglia.
Gupta, Deepak Prasad; Park, Sung Hee; Lee, Young-Sun; Lee, Sanghyun; Lim, Sujin; Byun, Jiin; Cho, Ik-Hyun; Song, Gyun Jee.
Afiliación
  • Gupta DP; Department of Medical Science, Catholic Kwandong University College of Medicine, Gangneung, Gangwon-do, Korea.
  • Park SH; Department of Medical Science, Catholic Kwandong University College of Medicine, Gangneung, Gangwon-do, Korea.
  • Lee YS; Department of Medical Science, Catholic Kwandong University College of Medicine, Gangneung, Gangwon-do, Korea; The Convergence Institute of Healthcare and Medical Science, Catholic Kwandong University, International St. Mary's Hospital, Incheon, Korea.
  • Lee S; Department of Plant Science and Technology, Chung-Ang University, Anseong, Korea.
  • Lim S; Department of Life Science, The Catholic University of Korea, Bucheon, Korea.
  • Byun J; Department of Life Science, The Catholic University of Korea, Bucheon, Korea.
  • Cho IH; Department of Convergence Medical Science, College of Korean Medicine, Kyung Hee University, Seoul, Korea. Electronic address: ihcho@khu.ac.kr.
  • Song GJ; Department of Medical Science, Catholic Kwandong University College of Medicine, Gangneung, Gangwon-do, Korea; The Convergence Institute of Healthcare and Medical Science, Catholic Kwandong University, International St. Mary's Hospital, Incheon, Korea. Electronic address: gjsong@cku.ac.kr.
Phytomedicine ; 108: 154486, 2023 Jan.
Article en En | MEDLINE | ID: mdl-36240609
BACKGROUND: Microglia are innate immune cells in the central nervous system that play a crucial role in neuroprotection by releasing neurotrophic factors, removing pathogens through phagocytosis, and regulating brain homeostasis. The constituents extracted from the roots and stems of the Daphne genkwa plant have shown neuroprotective effects in an animal model of Parkinson's disease. However, the effect of Daphne genkwa plant extract on microglia has yet to be demonstrated. PURPOSE: To study the anti-inflammatory and neuroprotective effects of Daphne genkwa flower extract (GFE) in microglia and explore the underlying mechanisms. METHODS: In-vitro mRNA expression levels of tumor necrosis factor-α (TNF-α), interleukin-1ß (IL-1ß), inducible nitric oxide synthase, Arginase1, and brain derived neurotropic factor (BDNF) were analyzed by reverse transcription polymerase chain reaction in microglia cells. Nitric oxide (NO) and TNF-α protein were respectively analyzed by Griess reagent and Enzyme Linked Immunosorbent Assay. Immunoreactivity of Iba-1, Neu-N, and BDNF in mouse brain were analyzed by immunofluorescence staining. Phagocytosis capacity of microglia was examined using fluorescent zymosan-red particles. RESULTS: GFE significantly inhibited lipopolysaccharide (LPS)-induced neuroinflammation and promoted neuroprotection both in vitro and in vivo. First, GFE inhibited the LPS-induced inflammatory factors NO, iNOS, and TNF-α in microglial cell lines and primary glial cells, thus demonstrating anti-inflammatory effects. Arginase1 and BDNF mRNA levels were increased in primary glial cells treated with GFE. Phagocytosis was also increased in microglia treated with GFE, suggesting a neuroprotective effect of GFE. In vivo, neuroprotective and anti-neuroinflammatory effects of GFE were also found in the mouse brain, as oral administration of GFE significantly inhibited LPS-induced neuronal loss and inflammatory activation of microglia. CONCLUSION: GFE has anti-inflammatory effects and promotes microglial neuroprotective effects. GFE inhibited the pro-inflammatory mediators and enhanced neuroprotective microglia activity by increasing BDNF expression and phagocytosis. These novel findings of the GFE effect on microglia show an innovative approach that can potentially promote neuroprotection for the prevention of neurodegenerative diseases.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Extractos Vegetales / Fármacos Neuroprotectores / Daphne Límite: Animals Idioma: En Revista: Phytomedicine Asunto de la revista: TERAPIAS COMPLEMENTARES Año: 2023 Tipo del documento: Article Pais de publicación: Alemania

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Extractos Vegetales / Fármacos Neuroprotectores / Daphne Límite: Animals Idioma: En Revista: Phytomedicine Asunto de la revista: TERAPIAS COMPLEMENTARES Año: 2023 Tipo del documento: Article Pais de publicación: Alemania