Rotavirus quantification in children with acute gastroenteritis.
Intervirology
; 2022 Oct 14.
Article
en En
| MEDLINE
| ID: mdl-36244340
ABSTRACT
INTRODUCTION:
Most intractable diarrheas are treated with antibiotics, irrespective of the causative agent. This study aims to quantified Rotavirus with taqman real-time PCR in fecal samples of children with acute gastroenteritis in accordance with the program of reduction of drug-resistance and use of antibiotics.METHODS:
A total of 190 fecal specimens were collected from under-five-year-old children with acute gastroenteritis in pediatric Hospital Regina Margherita of Turin in Italy in 2017. A total of 38 out of 67 (56.7%) episodes of acute gastroenteritis were associated with Rotavirus genomic detection with multiplex commercial kit.RESULTS:
All fecal specimens were tested for the presence of RV and other GE viruses. The most commonly detected virus was norovirus (41%), astrovirus (15.8%), human bocavirus (8.9%), sapovirus (7.9%), human parechovirus (5.8%), rhinovirus (4.2%), and adenovirus (1%). In 66 out of 190 (34.7%) Rotavirus were detectable with the median viral load 7.2E+11±60E+11genomes/mg fecal specimens. DISCUSSION/CONCLUSIONS:
Our results showed that RV was present in around 34.7% of children hospitalized for AGE, a rate similar to those reported in previous studies conducted elsewhere which were in the range of 33-75%. Our protocol are able to quantify the absolute number of viral particle/mg of feces. The clinical utility of quantitative molecular assays, such as Rotavirus viral load, will be markedly improved.
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Colección:
01-internacional
Base de datos:
MEDLINE
Tipo de estudio:
Guideline
Idioma:
En
Revista:
Intervirology
Año:
2022
Tipo del documento:
Article