Cloning BRD4 long isoform into overexpression vectors for stable overexpression of BRD4-L in mammalian cells.
STAR Protoc
; 3(4): 101785, 2022 12 16.
Article
en En
| MEDLINE
| ID: mdl-36317179
ABSTRACT
Molecular cloning of BRD4-L is a challenging technique, because the DNA insert is formed by a long, GC-rich sequence, which folds into secondary structures. The present protocol defines a specific strategy to amplify BRD4-L, followed by the successful cloning of the gene into an overexpression vector. Since there are no existing protocols nor commercially available plasmids, this work provides a useful tool for studies involving molecular cloning of BRD4-L and could potentially be applied to other challenging genes.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Proteínas Nucleares
/
Vectores Genéticos
Límite:
Animals
Idioma:
En
Revista:
STAR Protoc
Año:
2022
Tipo del documento:
Article