High-throughput genetic screening of meiotic commitment using fluorescence microscopy in Saccharomyces cerevisiae.
STAR Protoc
; 3(4): 101797, 2022 12 16.
Article
en En
| MEDLINE
| ID: mdl-36325582
ABSTRACT
Simple genetic screens in budding yeast have identified many conserved meiotic regulators. However, the identification of genes involved in specific steps of meiosis may require a more complex genetic screen that allows visualization of meiosis. Here, we describe a high-throughput protocol using fluorescence microscopy to systematically screen an overexpression library to identify genes involved in meiotic commitment. We also explain how this protocol can be adapted for identifying proteins that function at different stages of meiosis. For complete details on the use and execution of this protocol, please refer to Gavade et al. (2022).
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Saccharomyces cerevisiae
/
Proteínas de Saccharomyces cerevisiae
Tipo de estudio:
Diagnostic_studies
/
Prognostic_studies
/
Screening_studies
Idioma:
En
Revista:
STAR Protoc
Año:
2022
Tipo del documento:
Article