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Generation and characterization of a human induced pluripotent stem cell (iPSC) line from a patient with congenital heart disease (CHD).
Lin, Hui; Ye, Shi-Qiao; Xu, Zhao-Hui; Penaloza, Jacqueline S; Aljuhani, Mona; Vetter, Tatyana; Zhao, Ming-Tao; McBride, Kim L.
Afiliación
  • Lin H; Center for Cardiovascular Research and The Heart Center, Nationwide Children's Hospital, Columbus, OH, USA.
  • Ye SQ; Center for Cardiovascular Research and The Heart Center, Nationwide Children's Hospital, Columbus, OH, USA.
  • Xu ZH; Center for Vaccines and Immunity, Nationwide Children's Hospital, Columbus, OH, USA; Department of Pediatrics, College of Medicine, The Ohio State University, Columbus, OH, USA.
  • Penaloza JS; Computational Genomics Group, The Steve and Cindy Rasmussen Institute for Genomic Medicine, Nationwide Children's Hospital, Columbus, OH, USA.
  • Aljuhani M; Center for Cardiovascular Research and The Heart Center, Nationwide Children's Hospital, Columbus, OH, USA.
  • Vetter T; Center for Gene Therapy, Nationwide Children's Hospital, Columbus, OH, USA.
  • Zhao MT; Center for Cardiovascular Research and The Heart Center, Nationwide Children's Hospital, Columbus, OH, USA; Department of Pediatrics, College of Medicine, The Ohio State University, Columbus, OH, USA.
  • McBride KL; Center for Cardiovascular Research and The Heart Center, Nationwide Children's Hospital, Columbus, OH, USA; Department of Pediatrics, College of Medicine, The Ohio State University, Columbus, OH, USA. Electronic address: Kim.mcbride@nationwidechildrens.org.
Stem Cell Res ; 65: 102958, 2022 12.
Article en En | MEDLINE | ID: mdl-36343514
ABSTRACT
Epstein-Barr virus (EBV) immortalized lymphoblastoid cell lines (LCLs) are widely used for banking. This bioresource could be leveraged for creating human iPSC lines to model diseases including CHD. We generated an LCL-derived iPSC line (NCHi001-A) from a patient with congenital aortic valve stenosis. NCHi001-A was EBV and transgenes free, exhibited stem cell-like morphology, expressed pluripotency markers, has a normal karyotype, and could be differentiated into cells of three germ layers in vitro. Relationship inference via a microarray-based analysis showed NCHi001-A is identical to the parental cell line. NCHi001-A can be used for disease modeling, drug discovery, and cell therapy development.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Infecciones por Virus de Epstein-Barr / Células Madre Pluripotentes Inducidas / Cardiopatías Congénitas Límite: Humans Idioma: En Revista: Stem Cell Res Año: 2022 Tipo del documento: Article País de afiliación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Infecciones por Virus de Epstein-Barr / Células Madre Pluripotentes Inducidas / Cardiopatías Congénitas Límite: Humans Idioma: En Revista: Stem Cell Res Año: 2022 Tipo del documento: Article País de afiliación: Estados Unidos