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Transcriptome analysis of response strategy in Hemerocallis fulva under drought stress.
Cai, Xiaoteng; Liu, Jialin; Zhao, Fukuan; Wang, Xiaoqin.
Afiliación
  • Cai X; Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing University of Agriculture, Beijing, 102206, People's Republic of China.
  • Liu J; College of Bioscience and Resources Environment, Beijing University of Agriculture, Beijing, 102206, People's Republic of China.
  • Zhao F; Key Laboratory of Urban Agriculture in North China, Ministry of Agriculture and Rural Affairs, Beijing University of Agriculture, Beijing, 102206, People's Republic of China. 19966201@bua.edu.cn.
  • Wang X; College of Bioscience and Resources Environment, Beijing University of Agriculture, Beijing, 102206, People's Republic of China. 19966201@bua.edu.cn.
Genes Genomics ; 45(5): 593-610, 2023 05.
Article en En | MEDLINE | ID: mdl-36348249
BACKGROUND: Hemerocallis fulva is an important ground cover plant widely used in urban greening. The analysis of the molecular mechanism underlying the drought response of H. fulva can lay a foundation for improving its adaptability and expanding its planting area. OBJECTIVE: To reveal the drought response mechanisms of H. fulva, identify candidate unigenes associated with drought response, and lay a foundation for further unigenes functional study and drought resistance improvement of H. fulva via genetic engineering. METHODS: RNA was isolated from H. fulva under different experimental conditions. De novo transcriptomic analysis of the samples was performed to screen drought response unigenes. The transcriptional changes of candidate drought response unigenes were verified by quantitative real-time PCR. RESULTS: The differentially expressed unigenes and their functions were analyzed after H. fulva treated by PEG-simulated drought stress and rewatering. The candidate unigenes, associated with H. fulva drought response, were identified after transcriptome analysis. Then, the transcription level of drought response unigenes of H. fulva under different conditions was further verified. Abscisic acid, protein phosphorylation, sterol biosynthesis and ion transport were involved in drought response with quick restore in H. fulva. The response unigenes, involved in hormone (ABA, JA, CK and GA) signaling pathways, defense response, high light response, karrikin response and leaf shaping, can maintain at changed expression levels even after stress withdraw. CONCLUSION: Hemerocallis fulva has unique drought response mechanism. Negative regulation mechanism may play more important roles in drought response of H. fulva. The analysis of candidate unigenes, associated with drought response, lays a foundation for further drought resistance improvement of H. fulva.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Hemerocallis / Transcriptoma Idioma: En Revista: Genes Genomics Año: 2023 Tipo del documento: Article Pais de publicación: Corea del Sur

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Hemerocallis / Transcriptoma Idioma: En Revista: Genes Genomics Año: 2023 Tipo del documento: Article Pais de publicación: Corea del Sur