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Protein-free, ultrasensitive miRNA analysis based on an entropy-driven catalytic reaction switched on a smart-responsive DNAzyme dual-walker amplification strategy.
Fan, Zhichao; Zhao, Xiang; Dong, Yan; Zhou, Jie; Li, Yingxue; Wang, Junyi; Qi, Yuchen; Tan, Congcong; Yu, Hua; Li, Jianjun.
Afiliación
  • Fan Z; School of Medical and Life Sciences, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China; Department of Oncology, Southwest Hospital, Third Military Medical University (Army Medical University), Chongqing 400038, China.
  • Zhao X; Department of Oncology, Southwest Hospital, Third Military Medical University (Army Medical University), Chongqing 400038, China. Electronic address: zhaoxiang@tmmu.edu.cn.
  • Dong Y; Department of Oncology, Southwest Hospital, Third Military Medical University (Army Medical University), Chongqing 400038, China.
  • Zhou J; Department of Laboratory Medicine, Xingcheng Special Service Sanatorium of Strategic Support Force, Huludao 125100, China.
  • Li Y; School of Medical and Life Sciences, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China.
  • Wang J; Department of Oncology, Southwest Hospital, Third Military Medical University (Army Medical University), Chongqing 400038, China.
  • Qi Y; School of Medical and Life Sciences, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China; Department of Oncology, Southwest Hospital, Third Military Medical University (Army Medical University), Chongqing 400038, China.
  • Tan C; School of Medical and Life Sciences, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China; Department of Oncology, Southwest Hospital, Third Military Medical University (Army Medical University), Chongqing 400038, China.
  • Yu H; School of Medical and Life Sciences, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China; Department of General Surgery, Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu 610072, China. Electronic address: yuhua1232021@163.com.
  • Li J; Department of Oncology, Southwest Hospital, Third Military Medical University (Army Medical University), Chongqing 400038, China. Electronic address: jianjunli@tmmu.edu.cn.
Int J Biol Macromol ; 223(Pt A): 931-938, 2022 Dec 31.
Article en En | MEDLINE | ID: mdl-36372107
MicroRNAs (miRNAs), useful biomarkers for cancer diagnosis, play an important role in tumorigenesis and progression, but many of the current analysis methods can suffer from excessive protease dependence, being time-consuming and unsatisfactory performance. Therefore, a reliable sensing strategy for the protein-free, ultrasensitive analysis of tumor-associated miRNAs is desired. The proposed dual-walker biosensing strategy based on an entropy-driven catalytic (EDC) walker coupled with a smart-responsive DNAzyme walker was demonstrated for the dual-amplification detection of miRNA-21. Namely, the target miRNA-21 initiates the three-stranded substrate complex of the traditional EDC circuit to release the input trigger of the Dz walker, which recognizes the circular binding domain to restore the cleavage activity of the DzS-AuNP walker. The fluorescence signal continuously released from the AuNPs was recorded by a fluorescence reader for miRNA-21 sensing. The optimized dual-walker exhibited appreciable sensitivity with a detection limit of 70 fM, satisfactory flexibility, fine specificity and ideal stability for clinical serum sample assays. The proposed strategy may open a new avenue for the development of powerful DNA molecular tools for cancer diagnosis.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Técnicas Biosensibles / ADN Catalítico / MicroARNs / Nanopartículas del Metal / Neoplasias Límite: Humans Idioma: En Revista: Int J Biol Macromol Año: 2022 Tipo del documento: Article País de afiliación: China Pais de publicación: Países Bajos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Técnicas Biosensibles / ADN Catalítico / MicroARNs / Nanopartículas del Metal / Neoplasias Límite: Humans Idioma: En Revista: Int J Biol Macromol Año: 2022 Tipo del documento: Article País de afiliación: China Pais de publicación: Países Bajos