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Rapid quantification of alcohol production in microorganisms based on nanostructure-initiator mass spectrometry (NIMS).
Deng, Kai; Wang, Xi; Ing, Nicole; Opgenorth, Paul; de Raad, Markus; Kim, Jinho; Simmons, Blake A; Adams, Paul D; Singh, Anup K; Lee, Taek Soon; Northen, Trent R.
Afiliación
  • Deng K; Joint BioEnergy Institute, Emeryville, CA, 94608, USA; Sandia National Laboratories, Livermore, CA, 94551, USA. Electronic address: kdeng@sandia.gov.
  • Wang X; Joint BioEnergy Institute, Emeryville, CA, 94608, USA; Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.
  • Ing N; Joint BioEnergy Institute, Emeryville, CA, 94608, USA; Sandia National Laboratories, Livermore, CA, 94551, USA.
  • Opgenorth P; Joint BioEnergy Institute, Emeryville, CA, 94608, USA; Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.
  • de Raad M; Joint BioEnergy Institute, Emeryville, CA, 94608, USA; Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.
  • Kim J; Joint BioEnergy Institute, Emeryville, CA, 94608, USA; Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.
  • Simmons BA; Joint BioEnergy Institute, Emeryville, CA, 94608, USA; Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.
  • Adams PD; Joint BioEnergy Institute, Emeryville, CA, 94608, USA; Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA; University of California, Berkeley, CA, 94720, USA.
  • Singh AK; Joint BioEnergy Institute, Emeryville, CA, 94608, USA; Lawrence Livermore National Laboratory, Livermore, 94550, USA.
  • Lee TS; Joint BioEnergy Institute, Emeryville, CA, 94608, USA; Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.
  • Northen TR; Joint BioEnergy Institute, Emeryville, CA, 94608, USA; Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA. Electronic address: trnorthen@lbl.gov.
Anal Biochem ; 662: 114997, 2023 02 01.
Article en En | MEDLINE | ID: mdl-36435200
We described a mass spectrometry-based assay to rapidly quantify the production of primary alcohols directly from cell cultures. This novel assay used the combination of TEMPO-based oxidation chemistry and oxime ligation, followed by product analysis based on Nanostructure-Initiator Mass Spectrometry. This assay enables quantitative monitor both C5 to C18 alcohols as well as glucose and gluconate in the growth medium to support strain characterization and optimization. We find that this assay yields similar results to gas chromatography for isoprenol production but required much less acquisition time per sample. We applied this assay to gain new insights into P. Putida's utilization of alcohols and find that this strain largely could not grow on heptanol and octanol.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Nanoestructuras Idioma: En Revista: Anal Biochem Año: 2023 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Nanoestructuras Idioma: En Revista: Anal Biochem Año: 2023 Tipo del documento: Article Pais de publicación: Estados Unidos