Rapid quantification of alcohol production in microorganisms based on nanostructure-initiator mass spectrometry (NIMS).
Anal Biochem
; 662: 114997, 2023 02 01.
Article
en En
| MEDLINE
| ID: mdl-36435200
We described a mass spectrometry-based assay to rapidly quantify the production of primary alcohols directly from cell cultures. This novel assay used the combination of TEMPO-based oxidation chemistry and oxime ligation, followed by product analysis based on Nanostructure-Initiator Mass Spectrometry. This assay enables quantitative monitor both C5 to C18 alcohols as well as glucose and gluconate in the growth medium to support strain characterization and optimization. We find that this assay yields similar results to gas chromatography for isoprenol production but required much less acquisition time per sample. We applied this assay to gain new insights into P. Putida's utilization of alcohols and find that this strain largely could not grow on heptanol and octanol.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Nanoestructuras
Idioma:
En
Revista:
Anal Biochem
Año:
2023
Tipo del documento:
Article
Pais de publicación:
Estados Unidos