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c-Src activation as a potential marker of chemical-induced skin irritation using tissue-engineered skin equivalents.
Harding, Amy L; Colley, Helen E; Vazquez, Inmaculada Barragan; Danby, Simon; Hasan, Md Zobaer; Nakanishi, Hirofumi; Furuno, Tetsuo; Murdoch, Craig.
Afiliación
  • Harding AL; School of Clinical Dentistry, University of Sheffield, Sheffield, UK.
  • Colley HE; School of Clinical Dentistry, University of Sheffield, Sheffield, UK.
  • Vazquez IB; School of Clinical Dentistry, University of Sheffield, Sheffield, UK.
  • Danby S; Sheffield Dermatology Research, Department of Infection, Immunity & Cardiovascular Disease, Medical School, University of Sheffield, Sheffield, UK.
  • Hasan MZ; Rohto Pharmaceutical Co., Ltd., Safety Design Centre, Kyoto, Japan.
  • Nakanishi H; Rohto Pharmaceutical Co., Ltd., Safety Design Centre, Kyoto, Japan.
  • Furuno T; Rohto Pharmaceutical Co., Ltd., Safety Design Centre, Kyoto, Japan.
  • Murdoch C; School of Clinical Dentistry, University of Sheffield, Sheffield, UK.
Exp Dermatol ; 32(2): 220-225, 2023 02.
Article en En | MEDLINE | ID: mdl-36457227
Skin irritancy to topically applied chemicals is a significant problem that affects millions of people worldwide. New or modified chemical entities must be tested for potential skin irritancy by industry as part of the safety and toxicity profiling process. Many of these tests have now moved to a non-animal-based format to reduce experiments on animals. However, these tests for irritancy potential often rely on monolayer cultures of keratinocytes that are not representative of the skin architecture or tissue-engineered human skin equivalents (HSE) using complex multi-gene expression panels that are often cumbersome and not amenable for high throughput. Here, we show that human skin equivalents increase abundance of several phosphorylated kinases (c-Src, c-Jun, p53, GSK3α/ß) in response to irritant chemical stimulation by phosphokinase array analysis. Specific phosphorylation of c-SrcY419 was confirmed by immunoblotting and was plasma membrane-associated in basal/spinous cells by phospho-specific immunohistochemistry. Moreover, c-SrcY419 phosphorylation in response to the irritants lactic acid and capsaicin was inhibited by the c-Src inhibitors KB-SRC and betaine trimethylglycine. These data provide the first evidence for c-Src specific activation in response to chemical irritants and point to the development of new modes of rapid testing by immunodetection for first-pass screening of potential irritants.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Enfermedades de la Piel / Irritantes Límite: Animals / Humans Idioma: En Revista: Exp Dermatol Asunto de la revista: DERMATOLOGIA Año: 2023 Tipo del documento: Article Pais de publicación: Dinamarca

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Enfermedades de la Piel / Irritantes Límite: Animals / Humans Idioma: En Revista: Exp Dermatol Asunto de la revista: DERMATOLOGIA Año: 2023 Tipo del documento: Article Pais de publicación: Dinamarca