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Targeted phospholipidomic analysis of synovial fluid as a tool for osteoarthritis deep phenotyping.
Rocha, Beatriz; Illiano, Anna; Calamia, Valentina; Pinto, Gabriella; Amoresano, Angela; Ruiz-Romero, Cristina; Blanco, Francisco J.
Afiliación
  • Rocha B; Unidad de Proteómica. Grupo de Investigación de Reumatología (GIR, Instituto de Investigación Biomédica de A Coruña (INIBIC), Complexo Hospitalario Universitario de A Coruña (CHUAC), Sergas. Universidade da Coruña (UDC), C/ As Xubias de Arriba 84, 15006, A Coruña, Spain.
  • Illiano A; CEINGE - Advanced Biotechnology, 80145, Napoli, Italy.
  • Calamia V; Unidad de Proteómica. Grupo de Investigación de Reumatología (GIR, Instituto de Investigación Biomédica de A Coruña (INIBIC), Complexo Hospitalario Universitario de A Coruña (CHUAC), Sergas. Universidade da Coruña (UDC), C/ As Xubias de Arriba 84, 15006, A Coruña, Spain.
  • Pinto G; Department of Chemical Sciences, University of Naples "Federico II", Napoli, Italy.
  • Amoresano A; Department of Chemical Sciences, University of Naples "Federico II", Napoli, Italy.
  • Ruiz-Romero C; Unidad de Proteómica. Grupo de Investigación de Reumatología (GIR, Instituto de Investigación Biomédica de A Coruña (INIBIC), Complexo Hospitalario Universitario de A Coruña (CHUAC), Sergas. Universidade da Coruña (UDC), C/ As Xubias de Arriba 84, 15006, A Coruña, Spain.
  • Blanco FJ; Centro de Investigación Biomédica en Red de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Av. Monforte de Lemos, 3-5. Pabellón 11, 28029, Madrid, Spain.
Osteoarthr Cartil Open ; 3(4): 100219, 2021 Dec.
Article en En | MEDLINE | ID: mdl-36474758
ABSTRACT

Objective:

The aim of this study was to carry out a targeted phospholipidomic analysis on synovial fluid (SF) from patients with different grades of osteoarthritis (OA) and controls, in order to search for specific phospholipid profiles that may be useful for the deep phenotyping of this disease.

Design:

Multiple reaction monitoring-mass spectrometry (MRM/MS) was applied to explore the potential phospholipidomic differences in the SF of knee OA patients (n â€‹= â€‹15) (subclassified into early- and late-stage OA) and non-OA controls (n â€‹= â€‹4). Multivariate statistical analyses conducted by partial least squares discriminant analysis (PLS-DA) and hierarchical clustering analysis (HCA) were performed to identify significantly altered phospholipids in OA, characterize phospholipidomic profiles associated with the radiographic stage of the disease and describe potential endotypes at early stages.

Results:

Significant discrimination of phospholipid profiles between non-OA controls and the early- and late-stage OA groups were found by PLS-DA and HCA. Compared to SF from non-OA controls, OA patients showed higher levels of most quantified phospholipid species, including phosphatidylcholines (PC), phosphatidylserines and phosphatidylinositols. Furthermore, several PC species showed significant differences in abundance between the two OA subgroups and were negatively correlated with cartilage damage. Finally, two distinct endotypes of early-stage OA were identified based on the phospholipidomic profile of SF.

Conclusions:

Our data provides a novel insight into the phospholipid profiles of OA synovial fluid, revealing specific alterations associated with the radiographic stage of the disease. This targeted phospholipidomic profiling also facilitated the characterization of two different OA endotypes at early stages of the disease.
Palabras clave

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Osteoarthr Cartil Open Año: 2021 Tipo del documento: Article País de afiliación: España

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Osteoarthr Cartil Open Año: 2021 Tipo del documento: Article País de afiliación: España