Construction of an experimental study and addition of adapter sequences using HiDi DNA polymerase for improving DNA normalization methods relevant to novel gene discovery.

Uenosono, Yuya; Kawakami, Ryohei; Matsumoto, Shogo; Yamaguchi, Yoshihiro

Uenosono, Yuya; Kawakami, Ryohei; Matsumoto, Shogo; Yamaguchi, Yoshihiro.

Afiliación

Uenosono Y; Department of Materials Science and Applied Chemistry, Graduate School of Science and Technology, Kumamoto University, 2-39-1 Kurokami Chuo-ku, Kumamoto 860-8555, Japan.
Kawakami R; Department of Materials Science and Applied Chemistry, Graduate School of Science and Technology, Kumamoto University, 2-39-1 Kurokami Chuo-ku, Kumamoto 860-8555, Japan.
Matsumoto S; Department of Materials Science and Applied Chemistry, Graduate School of Science and Technology, Kumamoto University, 2-39-1 Kurokami Chuo-ku, Kumamoto 860-8555, Japan.
Yamaguchi Y; Department of Materials Science and Applied Chemistry, Graduate School of Science and Technology, Kumamoto University, 2-39-1 Kurokami Chuo-ku, Kumamoto 860-8555, Japan; Environmental Safety Center, Kumamoto University, 2-40-1 Kurokami, Chuo-ku, Kumamoto 860-8555, Japan. Electronic address: yyamagu@

J Microbiol Methods ; 204: 106631, 2023 01.

Article en En | MEDLINE | ID: mdl-36503828

Asunto(s)

ADN Polimerasa Dirigida por ADN; ADN; ADN/genética; Oligonucleótidos; Estudios de Asociación Genética

Palabras clave

DNA normalization; Experimental study; Function-based screening; HiDi DNA polymerase; Metagenome; Rare gene

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: ADN / ADN Polimerasa Dirigida por ADN Idioma: En Revista: J Microbiol Methods Año: 2023 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Países Bajos

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