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Rapid Point-of-Care Assay by SERS Detection of SARS-CoV-2 Virus and Its Variants.
Guan, Peng-Cheng; Zhang, Hong; Li, Zhi-Yong; Xu, Shan-Shan; Sun, Miao; Tian, Xian-Min; Ma, Zhan; Lin, Jia-Sheng; Gu, Man-Man; Wen, Huan; Zhang, Fan-Li; Zhang, Yue-Jiao; Yu, Guang-Jun; Yang, Chaoyong; Wang, Zhan-Xiang; Song, Yanling; Li, Jian-Feng.
Afiliación
  • Guan PC; College of Materials, State Key Laboratory for Physical Chemistry of Solid Surfaces, iChEM, MOE Key Laboratory of Spectrochemical Analysis and Instrumentation, College of Chemistry and Chemical Engineering, College of Energy, The First Affiliated Hospital, Xiamen University, Xiamen 361005, China.
  • Zhang H; Shanghai Children's Hospital, Shanghai Jiao Tong University, Shanghai 200062, China.
  • Li ZY; College of Materials, State Key Laboratory for Physical Chemistry of Solid Surfaces, iChEM, MOE Key Laboratory of Spectrochemical Analysis and Instrumentation, College of Chemistry and Chemical Engineering, College of Energy, The First Affiliated Hospital, Xiamen University, Xiamen 361005, China.
  • Xu SS; College of Materials, State Key Laboratory for Physical Chemistry of Solid Surfaces, iChEM, MOE Key Laboratory of Spectrochemical Analysis and Instrumentation, College of Chemistry and Chemical Engineering, College of Energy, The First Affiliated Hospital, Xiamen University, Xiamen 361005, China.
  • Sun M; College of Materials, State Key Laboratory for Physical Chemistry of Solid Surfaces, iChEM, MOE Key Laboratory of Spectrochemical Analysis and Instrumentation, College of Chemistry and Chemical Engineering, College of Energy, The First Affiliated Hospital, Xiamen University, Xiamen 361005, China.
  • Tian XM; Shanghai Children's Hospital, Shanghai Jiao Tong University, Shanghai 200062, China.
  • Ma Z; Shanghai Children's Hospital, Shanghai Jiao Tong University, Shanghai 200062, China.
  • Lin JS; College of Materials, State Key Laboratory for Physical Chemistry of Solid Surfaces, iChEM, MOE Key Laboratory of Spectrochemical Analysis and Instrumentation, College of Chemistry and Chemical Engineering, College of Energy, The First Affiliated Hospital, Xiamen University, Xiamen 361005, China.
  • Gu MM; Department of Optics and Electronic Technology, China Jiliang University, Hangzhou 310018, China.
  • Wen H; College of Materials, State Key Laboratory for Physical Chemistry of Solid Surfaces, iChEM, MOE Key Laboratory of Spectrochemical Analysis and Instrumentation, College of Chemistry and Chemical Engineering, College of Energy, The First Affiliated Hospital, Xiamen University, Xiamen 361005, China.
  • Zhang FL; Department of Optics and Electronic Technology, China Jiliang University, Hangzhou 310018, China.
  • Zhang YJ; College of Materials, State Key Laboratory for Physical Chemistry of Solid Surfaces, iChEM, MOE Key Laboratory of Spectrochemical Analysis and Instrumentation, College of Chemistry and Chemical Engineering, College of Energy, The First Affiliated Hospital, Xiamen University, Xiamen 361005, China.
  • Yu GJ; Shanghai Children's Hospital, Shanghai Jiao Tong University, Shanghai 200062, China.
  • Yang C; College of Materials, State Key Laboratory for Physical Chemistry of Solid Surfaces, iChEM, MOE Key Laboratory of Spectrochemical Analysis and Instrumentation, College of Chemistry and Chemical Engineering, College of Energy, The First Affiliated Hospital, Xiamen University, Xiamen 361005, China.
  • Wang ZX; Innovation Laboratory for Sciences and Technologies of Energy Materials of Fujian Province (IKKEM), Xiamen 361005, China.
  • Song Y; College of Materials, State Key Laboratory for Physical Chemistry of Solid Surfaces, iChEM, MOE Key Laboratory of Spectrochemical Analysis and Instrumentation, College of Chemistry and Chemical Engineering, College of Energy, The First Affiliated Hospital, Xiamen University, Xiamen 361005, China.
  • Li JF; College of Materials, State Key Laboratory for Physical Chemistry of Solid Surfaces, iChEM, MOE Key Laboratory of Spectrochemical Analysis and Instrumentation, College of Chemistry and Chemical Engineering, College of Energy, The First Affiliated Hospital, Xiamen University, Xiamen 361005, China.
Anal Chem ; 94(51): 17795-17802, 2022 12 27.
Article en En | MEDLINE | ID: mdl-36511436
ABSTRACT
Addressing the spread of coronavirus disease 2019 (COVID-19) has highlighted the need for rapid, accurate, and low-cost diagnostic methods that detect specific antigens for SARS-CoV-2 infection. Tests for COVID-19 are based on reverse transcription PCR (RT-PCR), which requires laboratory services and is time-consuming. Here, by targeting the SARS-CoV-2 spike protein, we present a point-of-care SERS detection platform that specifically detects SARS-CoV-2 antigen in one step by captureing substrates and detection probes based on aptamer-specific recognition. Using the pseudovirus, without any pretreatment, the SARS-CoV-2 virus and its variants were detected by a handheld Raman spectrometer within 5 min. The limit of detection (LoD) for the pseudovirus was 124 TU µL-1 (18 fM spike protein), with a linear range of 250-10,000 TU µL-1. Moreover, this assay can specifically recognize the SARS-CoV-2 antigen without cross reacting with specific antigens of other coronaviruses or influenza A. Therefore, the platform has great potential for application in rapid point-of-care diagnostic assays for SARS-CoV-2.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: SARS-CoV-2 / COVID-19 Tipo de estudio: Diagnostic_studies Límite: Humans Idioma: En Revista: Anal Chem Año: 2022 Tipo del documento: Article País de afiliación: China
Texto completo
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XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: SARS-CoV-2 / COVID-19 Tipo de estudio: Diagnostic_studies Límite: Humans Idioma: En Revista: Anal Chem Año: 2022 Tipo del documento: Article País de afiliación: China