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Efficient simultaneous double DNA knock-in in murine embryonic stem cells by CRISPR/Cas9 ribonucleoprotein-mediated circular plasmid targeting for generating gene-manipulated mice.
Ozawa, Manabu; Taguchi, Jumpei; Katsuma, Kento; Ishikawa-Yamauchi, Yu; Kikuchi, Mio; Sakamoto, Reiko; Yamada, Yasuhiro; Ikawa, Masahito.
Afiliación
  • Ozawa M; Laboratory of Reproductive Systems Biology, Center for Experimental Medicine and Systems Biology, The Institute of Medical Science, The University of Tokyo, Tokyo, 108-8639, Japan. semil@ims.u-tokyo.ac.jp.
  • Taguchi J; Division of Stem Cell Pathology, Center for Experimental Medicine and Systems Biology, The Institute of Medical Science, The University of Tokyo, Tokyo, 108-8639, Japan.
  • Katsuma K; Laboratory of Reproductive Systems Biology, Center for Experimental Medicine and Systems Biology, The Institute of Medical Science, The University of Tokyo, Tokyo, 108-8639, Japan.
  • Ishikawa-Yamauchi Y; Laboratory of Reproductive Systems Biology, Center for Experimental Medicine and Systems Biology, The Institute of Medical Science, The University of Tokyo, Tokyo, 108-8639, Japan.
  • Kikuchi M; Division of Stem Cell Pathology, Center for Experimental Medicine and Systems Biology, The Institute of Medical Science, The University of Tokyo, Tokyo, 108-8639, Japan.
  • Sakamoto R; Division of Stem Cell Pathology, Center for Experimental Medicine and Systems Biology, The Institute of Medical Science, The University of Tokyo, Tokyo, 108-8639, Japan.
  • Yamada Y; Division of Stem Cell Pathology, Center for Experimental Medicine and Systems Biology, The Institute of Medical Science, The University of Tokyo, Tokyo, 108-8639, Japan.
  • Ikawa M; Laboratory of Reproductive Systems Biology, Center for Experimental Medicine and Systems Biology, The Institute of Medical Science, The University of Tokyo, Tokyo, 108-8639, Japan. ikawa@biken.osaka-u.ac.jp.
Sci Rep ; 12(1): 21558, 2022 12 13.
Article en En | MEDLINE | ID: mdl-36513736
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ribonucleoproteínas / Sistemas CRISPR-Cas Límite: Animals Idioma: En Revista: Sci Rep Año: 2022 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Reino Unido
Texto completo
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Imprimir
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PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ribonucleoproteínas / Sistemas CRISPR-Cas Límite: Animals Idioma: En Revista: Sci Rep Año: 2022 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Reino Unido