Duck hepatitis A virus type 1 transmission by exosomes establishes a productive infection in vivo and in vitro.
Vet Microbiol
; 277: 109621, 2023 Feb.
Article
en En
| MEDLINE
| ID: mdl-36525908
ABSTRACT
Duck hepatitis A virus type 1 (DHAV-1) infection causes an acute and highly fatal disease in young ducklings. Exosomes are nano-sized small extracellular vesicles secreted by various cells, which participate in intercellular communication and play a key role in the physiological and pathological processes. However, the role of exosomes in DHAV-1 transmission remains unknown. In this study, through RT-PCR, WB analysis and TEM observation, the complete DHAV-1 genomic RNA, partial viral proteins, and virions were respectively identified in the exosomes derived from DHAV-1-infected duck embryo fibroblasts (DEFs). The productive DHAV-1 infection was transmitted by exosomes in DEFs, duck embryos, and ducklings, and high titers of neutralizing antibodies completely blocked DHAV-1 infection but did not significantly neutralize exosome-mediated DHAV-1 infection. To the best of our knowledge, this is the first report that exosome-mediated DHAV-1 infection was resistant to antibody neutralization in vivo and in vitro, which might be an immune evasion mechanism of DHAV-1.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Enfermedades de las Aves de Corral
/
Infecciones por Picornaviridae
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Virus de la Hepatitis del Pato
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Exosomas
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Hepatitis Viral Animal
Límite:
Animals
Idioma:
En
Revista:
Vet Microbiol
Año:
2023
Tipo del documento:
Article