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Live-cell super-resolution imaging of actin using LifeAct-14 with a PAINT-based approach.
Bhaskar, Haresh; Kleinjan, Dirk-Jan; Oi, Curran; Gidden, Zoe; Rosser, Susan J; Horrocks, Mathew H; Regan, Lynne.
Afiliación
  • Bhaskar H; The School of Biological Sciences, University of Edinburgh, Edinburgh, UK.
  • Kleinjan DJ; EaStCHEM School of Chemistry, University of Edinburgh, Edinburgh, UK.
  • Oi C; Centre for Synthetic and Systems Biology and UK Centre for Mammalian Synthetic Biology, School of Biological Sciences, University of Edinburgh, Edinburgh, UK.
  • Gidden Z; Department of Genome Sciences, University of Washington, Seattle, Washington, USA.
  • Rosser SJ; The School of Biological Sciences, University of Edinburgh, Edinburgh, UK.
  • Horrocks MH; EaStCHEM School of Chemistry, University of Edinburgh, Edinburgh, UK.
  • Regan L; Centre for Synthetic and Systems Biology and UK Centre for Mammalian Synthetic Biology, School of Biological Sciences, University of Edinburgh, Edinburgh, UK.
Protein Sci ; 32(2): e4558, 2023 02.
Article en En | MEDLINE | ID: mdl-36585831
ABSTRACT
We present direct-LIVE-PAINT, an easy-to-implement approach for the nanoscopic imaging of protein structures in live cells using labeled binding peptides. We demonstrate the feasibility of direct-LIVE-PAINT with an actin-binding peptide fused to EGFP, the location of which can be accurately determined as it transiently binds to actin filaments. We show that direct-LIVE-PAINT can be used to image actin structures below the diffraction-limit of light and have used it to observe the dynamic nature of actin in live cells. We envisage a similar approach could be applied to imaging other proteins within live mammalian cells.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Citoesqueleto de Actina / Actinas Límite: Animals Idioma: En Revista: Protein Sci Asunto de la revista: BIOQUIMICA Año: 2023 Tipo del documento: Article País de afiliación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Citoesqueleto de Actina / Actinas Límite: Animals Idioma: En Revista: Protein Sci Asunto de la revista: BIOQUIMICA Año: 2023 Tipo del documento: Article País de afiliación: Reino Unido