SART3 associates with a post-splicing complex.
J Cell Sci
; 136(2)2023 01 15.
Article
en En
| MEDLINE
| ID: mdl-36620952
ABSTRACT
SART3 is a multifunctional protein that acts in several steps of gene expression, including assembly and recycling of the spliceosomal U4/U6 small nuclear ribonucleoprotein particle (snRNP). In this work, we provide evidence that SART3 associates via its N-terminal HAT domain with the 12S U2 snRNP. Further analysis showed that SART3 associates with the post-splicing complex containing U2 and U5 snRNP components. In addition, we observed an interaction between SART3 and the RNA helicase DHX15, which disassembles post-splicing complexes. Based on our data, we propose a model that SART3 associates via its N-terminal HAT domain with the post-splicing complex, where it interacts with U6 snRNA to protect it and to initiate U6 snRNA recycling before a next round of splicing.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Empalme del ARN
/
Empalmosomas
Tipo de estudio:
Risk_factors_studies
Idioma:
En
Revista:
J Cell Sci
Año:
2023
Tipo del documento:
Article
País de afiliación:
República Checa