Detection and quantification of HBV and HCV in plasma samples by means of different molecular assays: Comparative study.
Enferm Infecc Microbiol Clin (Engl Ed)
; 42(1): 13-16, 2024 Jan.
Article
en En
| MEDLINE
| ID: mdl-36624027
ABSTRACT
INTRODUCTION:
Viral load is a very useful marker for monitoring patients infected with HBV and HCV. This work compares assays based on transcription-mediated amplification (TMA) and on real-time PCR (RT-PCR) to verify whether they can be interchangeable. MATERIAL ANDMETHODS:
A bicentric study, in which 147 plasma samples from patients infected with HBV and 229 with HCV were analyzed, was carried out. TMA-based assays (Aptima® HBV Quant and Aptima® HCV Quant Dx, employing Panther system (Hologic®)) and RT-PCR (COBAS® AmpliPrep/COBAS® TaqMan® and COBAS® 6800) were used and the degree of concordance between them was calculated.RESULTS:
Viral load was detected in both systems in 60 (40.82%) HBV samples (median log viral load COBAS 2.51IU/mL (IQR 2.20-3.17), Panther 2.71IU/mL (IQR 2.21-3.22)) and in 39 (16.96%) HCV samples (median log viral load COBAS 3.93IU/mL (IQR 2.24-6.01), Panther 3.80IU/mL (IQR 1.99-6.14)). The agreement between both systems was κ=0.943 for HBV and κ=0.925 for HCV. Comparison of viral load samples detected by both assays showed a hight correlation for HBV (R2=0.86) and for HCV (R2=0.97).CONCLUSIONS:
Both TMA and RT-PCR based assays may be interchangeable for the management of patients infected with HBV and HCV.Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Virus de la Hepatitis B
/
Hepatitis C
Tipo de estudio:
Diagnostic_studies
Límite:
Humans
Idioma:
En
Revista:
Enferm Infecc Microbiol Clin (Engl Ed)
Año:
2024
Tipo del documento:
Article