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Unraveling the effect of the inflammatory microenvironment in spermatogenesis progression.
Ferreiro, Maria Eugenia; Méndez, Cinthia Soledad; Glienke, Leilane; Sobarzo, Cristian Marcelo; Ferraris, María Jimena; Pisera, Daniel Alberto; Lustig, Livia; Jacobo, Patricia Verónica; Theas, María Susana.
Afiliación
  • Ferreiro ME; Queensland Brain Institute, University of Queensland, Brisbane, QLD, 4072, Australia.
  • Méndez CS; CONICET-Universidad de Buenos Aires, Instituto de Investigaciones Biomédicas (INBIOMED), Paraguay 2155, Piso 10, Laboratorio 7, Ciudad Autónoma de Buenos Aires, Buenos Aires, C1421ABG, Argentina.
  • Glienke L; Universidad de Buenos Aires, Facultad de Medicina, Departamento de Biología Celular, Cátedra II de Histología, Buenos Aires, Argentina.
  • Sobarzo CM; CONICET-Universidad de Buenos Aires, Instituto de Investigaciones Biomédicas (INBIOMED), Paraguay 2155, Piso 10, Laboratorio 7, Ciudad Autónoma de Buenos Aires, Buenos Aires, C1421ABG, Argentina.
  • Ferraris MJ; Universidad de Buenos Aires, Facultad de Medicina, Departamento de Biología Celular, Cátedra II de Histología, Buenos Aires, Argentina.
  • Pisera DA; CONICET-Universidad de Buenos Aires, Instituto de Investigaciones Biomédicas (INBIOMED), Paraguay 2155, Piso 10, Laboratorio 7, Ciudad Autónoma de Buenos Aires, Buenos Aires, C1421ABG, Argentina.
  • Lustig L; Department of Biochemistry and Biophysics, Stockholm University, Svante Arrhenius väg 16C SE-106 91, Stockholm, Sweden.
  • Jacobo PV; CONICET-Universidad de Buenos Aires, Instituto de Investigaciones Biomédicas (INBIOMED), Paraguay 2155, Piso 10, Laboratorio 7, Ciudad Autónoma de Buenos Aires, Buenos Aires, C1421ABG, Argentina.
  • Theas MS; Universidad de Buenos Aires, Facultad de Medicina, Departamento de Biología Celular, Cátedra II de Histología, Buenos Aires, Argentina.
Cell Tissue Res ; 392(2): 581-604, 2023 May.
Article en En | MEDLINE | ID: mdl-36627392
Experimental autoimmune orchitis (EAO) is a chronic inflammatory disorder that causes progressive spermatogenic impairment. EAO is characterized by high intratesticular levels of nitric oxide (NO) and tumor necrosis factor alpha (TNFα) causing germ cell apoptosis and Sertoli cell dysfunction. However, the impact of this inflammatory milieu on the spermatogenic wave is unknown. Therefore, we studied the effect of inflammation on spermatogonia and preleptotene spermatocyte cell cycle progression in an EAO context and through the intratesticular DETA-NO and TNFα injection in the normal rat testes. In EAO, premeiotic germ cell proliferation is limited as a consequence of the undifferentiated spermatogonia (CD9+) cell cycle arrest in G2/M and the reduced number of differentiated spermatogonia (c-kit+) and preleptotene spermatocytes that enter in the meiotic S-phase. Although inflammation disrupts spermatogenesis in EAO, it is maintained in some seminiferous tubules at XIV and VII-VIII stages of the epithelial cell cycle, thereby guaranteeing sperm production. We found that DETA-NO (2 mM) injected in normal testes arrests spermatogonia and preleptotene spermatocyte cell cycle; this effect reduces the number of proliferative spermatogonia and the number of preleptotene spermatocytes in meiosis S-phase (36 h after). The temporal inhibition of spermatogonia clonal amplification delayed progression of the spermatogenic wave (5 days after) finally altering spermatogenesis. TNFα (0.5 and 1 µg) exposure did not affect premeiotic germ cell cycle or spermatogenic wave. Our results show that in EAO the inflammatory microenvironment altered spermatogenesis kinetics through premeiotic germ cell cycle arrest and that NO is a sufficient factor contributing to this phenomenon.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Orquitis / Factor de Necrosis Tumoral alfa Límite: Animals / Humans / Male Idioma: En Revista: Cell Tissue Res Año: 2023 Tipo del documento: Article País de afiliación: Australia Pais de publicación: Alemania

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Orquitis / Factor de Necrosis Tumoral alfa Límite: Animals / Humans / Male Idioma: En Revista: Cell Tissue Res Año: 2023 Tipo del documento: Article País de afiliación: Australia Pais de publicación: Alemania