Development of a matrix-based candidate reference material for human glycated albumin using isotope-dilution liquid chromatography/tandem mass spectrometry.
Anal Chim Acta
; 1239: 340648, 2023 Jan 25.
Article
en En
| MEDLINE
| ID: mdl-36628699
ABSTRACT
Glycated albumin (GA) in human serum is tested clinically as a short-term indicator for glucose monitoring. Here, we evaluated a candidate serum reference material (RM) at three different GA concentrations to help standardize serum GA measurements. Both albumin and GA were quantitatively determined using isotope-dilution liquid chromatography/tandem mass spectrometry with lysine-4,4,5,5-D4·2HCl (D4-lysine) and Nε-l3C6-(l-deoxy-d-fructose-1-yl)-l-lysine (13C6-DOF-lysine) as internal standards and lysine and synthetic DOF-lysine as calibration standards. The method was evaluated with the RM, JCCRM611-1, from the Reference Material Institute for Clinical Chemistry Standards. The homogeneity and stability of the candidate RMs were examined using a commercial biochemical analyzer. Fifteen units were randomly selected, and statistical analysis showed no inhomogeneity. The candidate RMs were stable for at least 6 months at -80 °C. The coefficients of variation (CVs) for the JCCRM611-1 RM ranged from 3.2% to 2.3%, and the biases ranged from 4.12% to -1.84%. GA was tested at low, medium, and high concentrations, which were quantified as 249.53 ± 13.29, 408.02 ± 11.70, and 637.22 ± 17.03 mmol/mol, respectively. The overall CVs ranged from 0.99% to 2.51%. The candidate RMs can potentially be used to develop a traceability chain to improve the accuracy of GA measurements.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Espectrometría de Masas en Tándem
/
Lisina
Tipo de estudio:
Guideline
Límite:
Humans
Idioma:
En
Revista:
Anal Chim Acta
Año:
2023
Tipo del documento:
Article
País de afiliación:
China