Protocol for the induction of hindlimb ischemia and isolation of muscle endothelial cells in mice.
STAR Protoc
; 4(1): 102017, 2023 03 17.
Article
en En
| MEDLINE
| ID: mdl-36638013
ABSTRACT
A mouse model of hindlimb ischemia is an important tool for studying diverse therapeutic approaches for vascularization with high surgical success and low mortality rates. Here, we present a protocol for the induction of hindlimb ischemia in mice, including the surgery procedure and steps to analyze blood perfusion in the ischemic area using a laser speckle contrast analyzer. We also detail the isolation of endothelial cells from thigh muscles using flow cytometry after ischemic surgery. For complete details on the use and execution of this protocol, please refer to Park et al. (2016).1.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Neovascularización Fisiológica
/
Células Endoteliales
Tipo de estudio:
Guideline
Límite:
Animals
Idioma:
En
Revista:
STAR Protoc
Año:
2023
Tipo del documento:
Article
País de afiliación:
Estados Unidos