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Local and systemic targets of the MtCLE35-SUNN pathway in the roots of Medicago truncatula.
Lebedeva, M A; Dobychkina, D A; Yashenkova, Ya S; Romanyuk, D A; Lutova, L A.
Afiliación
  • Lebedeva MA; Department of Genetics and Biotechnology, Saint Petersburg State University, Universitetskaya emb.7/9, 199034, Saint Petersburg, Russia. Electronic address: m.a.lebedeva@spbu.ru.
  • Dobychkina DA; Department of Genetics and Biotechnology, Saint Petersburg State University, Universitetskaya emb.7/9, 199034, Saint Petersburg, Russia.
  • Yashenkova YS; Department of Genetics and Biotechnology, Saint Petersburg State University, Universitetskaya emb.7/9, 199034, Saint Petersburg, Russia.
  • Romanyuk DA; All-Russia Research Institute for Agricultural Microbiology (ARRIAM), Laboratory of Genetics of Plant-Microbe Interactions, Podbelsky Sh. 3, 196608, Saint-Petersburg, Russia.
  • Lutova LA; Department of Genetics and Biotechnology, Saint Petersburg State University, Universitetskaya emb.7/9, 199034, Saint Petersburg, Russia; Center for Genetic Technologies, N. I. Vavilov All-Russian Institute of Plant Genetic Resources (VIR), 190000 Saint Petersburg, Russia.
J Plant Physiol ; 281: 153922, 2023 Feb.
Article en En | MEDLINE | ID: mdl-36669364
CLE (CLAVATA3/ENDOSPERM SURROUNDING REGION-related) peptides are systemic regulators of legume-rhizobium symbiosis that negatively control the number of nitrogen-fixing nodules. CLE peptides are produced in the root in response to rhizobia inoculation and/or nitrate treatment and are transported to the shoot where they are recognized by the CLV1-like (CLAVATA1-like) receptor kinase. As a result, a shoot-derived signaling pathway is activated that inhibits subsequent nodule development in the root. In Medicago truncatula, MtCLE35 is activated in response to rhizobia and nitrate treatment and the overexpression of this gene systemically inhibits nodulation. The inhibitory effect of MtCLE35 overexpression is dependent on the CLV1-like receptor kinase MtSUNN (SUPER NUMERIC NODULES), suggesting that MtSUNN could be involved in the reception of the MtCLE35 peptide. Yet little is known about the downstream genes regulated by a MtCLE35-activated response in the root. In order to identify genes whose expression levels could be regulated by the MtCLE35-MtSUNN pathway, we performed a MACE-Seq (Massive Analysis of cDNA Ends) transcriptomic analysis of MtCLE35-overexpressing roots. Among upregulated genes, the gene MtSUNN that encodes a putative receptor of MtCLE35 was detected. Moreover, we found that MtSUNN, as well as several other differentially expressed genes, were upregulated locally in MtCLE35-overexpressing roots whereas the MtTML1 and MtTML2 genes were upregulated systemically. Our data suggest that MtCLE35 has both local and systemic effects on target genes in the root.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Medicago truncatula Idioma: En Revista: J Plant Physiol Asunto de la revista: BOTANICA Año: 2023 Tipo del documento: Article Pais de publicación: Alemania

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Medicago truncatula Idioma: En Revista: J Plant Physiol Asunto de la revista: BOTANICA Año: 2023 Tipo del documento: Article Pais de publicación: Alemania